Overview
- External ontology
- TOGO:M3248 — Basal salts solution supplemented with tetrathionate
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3248
Composition
flowchart LR
CultureMech_009680["basal_salts_solution_supplemented_with_tetrathionate"]
ing0_NH4Cl["NH4Cl"]
CultureMech_009680 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_K2HPO4["K2HPO4"]
CultureMech_009680 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_MgCl2_6H2O["MgCl2.6H2O"]
CultureMech_009680 --> ing2_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_6H2O -.-> CHEBI_86345
ing3_KCl["KCl"]
CultureMech_009680 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_Ca_NO3_2["Ca(NO3)2"]
CultureMech_009680 --> ing4_Ca_NO3_2
CHEBI_64205["CHEBI:64205"]
ing4_Ca_NO3_2 -.-> CHEBI_64205
ing5_Deionized_water["Deionized water"]
CultureMech_009680 --> ing5_Deionized_water
ing6_Tetrathionate["Tetrathionate"]
CultureMech_009680 --> ing6_Tetrathionate
CHEBI_15226["CHEBI:15226"]
ing6_Tetrathionate -.-> CHEBI_15226
ing7_HCl["HCl"]
CultureMech_009680 --> ing7_HCl
CHEBI_17883["CHEBI:17883"]
ing7_HCl -.-> CHEBI_17883
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.6 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
MgCl2.6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
Ca(NO3)2
Ca.2NO3 164.086 g/mol
|
0.01 G_PER_L |
CHEBI:64205
calcium nitrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 6 | Deionized water | 1 G_PER_L | Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound | |
| 7 |
Tetrathionate
O6S4 224.262 g/mol
|
20 MILLIMOLAR |
CHEBI:15226
tetrathionate(2-)
|
Properties: Defined component, Inorganic compound, Simple component |
| 8 |
HCl
HCl 36.461 g/mol
|
variable VARIABLE |
CHEBI:17883
hydrogen chloride
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.633550Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3248 |
| 2026-01-28T19:48:24.573204Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: HCl |
| 2026-03-13T06:20:08.061826Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009680 |
| 2026-03-17T06:30:17.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.066946+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Basal salts solution supplemented with tetrathionate" to "basal_salts_solution_supplemented_with_tetrathionate" for consistent matching |
| 2026-05-16T09:03:25.489652+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:50.569875+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:36.022811+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:53.816898+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-20T19:01:33.844691+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |