halobius_medium (CultureMech:009682)

Overview

External ontology

TOGO:M324 — Halobius Medium

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M324 Original source: JCM - JCM_M329 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=329

Composition

flowchart LR
    CultureMech_009682["halobius_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009682 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_009682 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_009682 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KCl["KCl"]
    CultureMech_009682 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_FeCl2_4H2O["FeCl2・4H2O"]
    CultureMech_009682 --> ing4_FeCl2_4H2O
    CHEBI_86249["CHEBI:86249"]
    ing4_FeCl2_4H2O -.-> CHEBI_86249
    ing5_Trisodium_citrate_2H2O["Trisodium citrate・2H2O"]
    CultureMech_009682 --> ing5_Trisodium_citrate_2H2O
    CHEBI_53258["CHEBI:53258"]
    ing5_Trisodium_citrate_2H2O -.-> CHEBI_53258
    ing6_Agar["Agar"]
    CultureMech_009682 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_009682 --> ing7_Yeast_extract__BD_Difco_
    ing8_Casamino_acids__vitamin_assay__BD_Difco_["Casamino acids, vitamin assay (BD-Difco)"]
    CultureMech_009682 --> ing8_Casamino_acids__vitamin_assay__BD_Difco_

Ingredients (9)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component
2	MgSO4・7H2O 7H2O.Mg.O4S 246.473 g/mol	20 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3	NaCl Cl.Na 58.443 g/mol	116 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
4	KCl Cl.K 74.551 g/mol	2 G_PER_L	CHEBI:32588 potassium chloride	Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
5	FeCl2・4H2O Cl2Fe.4H2O 198.811 g/mol	23 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate	Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
6	Trisodium citrate・2H2O C6H5O7.3Na 258.069 g/mol	3 G_PER_L	CHEBI:53258 sodium citrate	Role: Buffer; Properties: Simple component, Organic compound, Defined component
7	Agar	20 G_PER_L	CHEBI:2509 agar	Role: Solidifying component
8	Yeast extract (BD-Difco)	10 G_PER_L		Properties: Undefined component
9	Casamino acids, vitamin assay (BD-Difco)	7.5 G_PER_L		Properties: Undefined component

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:54.853850Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M324
2026-01-28T19:48:36.397145Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:08.073671Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009682
2026-03-17T06:30:42.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:27.077806+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Halobius Medium" to "halobius_medium" for consistent matching
2026-06-05T07:40:40.377035+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:29.701491+00:00	mgso4-heptahydrate-term-fix-v1.0	Corrected MgSO4·7H2O primary term grounding to heptahydrate	Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.