Overview
- External ontology
- TOGO:M3253 — MSF medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3253
Composition
flowchart LR
CultureMech_009685["msf_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009685 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_009685 --> ing1_Yeast_extract
ing2_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_009685 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_NH4Cl["NH4Cl"]
CultureMech_009685 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_NaMoO4_2H2O["NaMoO4·2H2O"]
CultureMech_009685 --> ing4_NaMoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing4_NaMoO4_2H2O -.-> CHEBI_75213
ing5_Resazurin["Resazurin"]
CultureMech_009685 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_009685 --> ing6_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_6H2O -.-> CHEBI_86345
ing7_Na2S_9H2O["Na2S·9H2O"]
CultureMech_009685 --> ing7_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_9H2O -.-> CHEBI_76209
ing8_H3BO3["H3BO3"]
CultureMech_009685 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_009685 --> ing9_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_7H2O -.-> CHEBI_75836
ing10_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_009685 --> ing10_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_4H2O -.-> CHEBI_86368
ing11_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_009685 --> ing11_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing11_CoCl2_6H2O -.-> CHEBI_35696
ing12_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_009685 --> ing12_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing12_CuCl2_2H2O -.-> CHEBI_86318
ing13_ZnCl2["ZnCl2"]
CultureMech_009685 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_Na2WO4_2H2O["Na2WO4·2H2O"]
CultureMech_009685 --> ing14_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing14_Na2WO4_2H2O -.-> CHEBI_63939
ing15_K2HPO4_3H2O["K2HPO4·3H2O"]
CultureMech_009685 --> ing15_K2HPO4_3H2O
CHEBI_131527["CHEBI:131527"]
ing15_K2HPO4_3H2O -.-> CHEBI_131527
ing16_NiSO4_6H2O["NiSO4·6H2O"]
CultureMech_009685 --> ing16_NiSO4_6H2O
CHEBI_53001["CHEBI:53001"]
ing16_NiSO4_6H2O -.-> CHEBI_53001
ing17_Mercaptoethanesulfonic_acid["Mercaptoethanesulfonic acid"]
CultureMech_009685 --> ing17_Mercaptoethanesulfonic_acid
ing18_AlCl3_6H2O["AlCl3·6H2O"]
CultureMech_009685 --> ing18_AlCl3_6H2O
CHEBI_30115["CHEBI:30115"]
ing18_AlCl3_6H2O -.-> CHEBI_30115
ing19_H2SeO3["H2SeO3"]
CultureMech_009685 --> ing19_H2SeO3
CHEBI_26642["CHEBI:26642"]
ing19_H2SeO3 -.-> CHEBI_26642
ing20_NaOH["NaOH"]
CultureMech_009685 --> ing20_NaOH
CHEBI_32145["CHEBI:32145"]
ing20_NaOH -.-> CHEBI_32145
ing21_Trypticase_peptone["Trypticase peptone"]
CultureMech_009685 --> ing21_Trypticase_peptone
ing22_Sodium_formate["Sodium formate"]
CultureMech_009685 --> ing22_Sodium_formate
CHEBI_62965["CHEBI:62965"]
ing22_Sodium_formate -.-> CHEBI_62965
ing23_Na2_EDTA_2H2O["Na2-EDTA·2H2O"]
CultureMech_009685 --> ing23_Na2_EDTA_2H2O
CHEBI_64758["CHEBI:64758"]
ing23_Na2_EDTA_2H2O -.-> CHEBI_64758
ing24_CO2["CO2"]
CultureMech_009685 --> ing24_CO2
CHEBI_16526["CHEBI:16526"]
ing24_CO2 -.-> CHEBI_16526
ing25_Hydrogen_gas["Hydrogen gas"]
CultureMech_009685 --> ing25_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing25_Hydrogen_gas -.-> CHEBI_18276
Ingredients (26)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 | Yeast extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.4 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NaMoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 7 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
|
0.25 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
1.5 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.2 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
1 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
Na2WO4·2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.3 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
K2HPO4·3H2O
HO4P.2K 174.174 g/mol
|
0.4 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
NiSO4·6H2O
Ni.O4S 154.756 g/mol
|
0.2 G_PER_L |
CHEBI:53001
nickel sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 | Mercaptoethanesulfonic acid | 0.5 G_PER_L | Role: Growth factor; Properties: Organic compound, Defined component, Simple component | |
| 19 |
AlCl3·6H2O
AlCl3.6H2O 241.431 g/mol
|
0.4 G_PER_L |
CHEBI:30115
aluminium trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
H2SeO3
H2O3Se 128.973 g/mol
|
0.1 G_PER_L |
CHEBI:26642
selenous acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
NaOH
HO.Na 39.997 g/mol
|
4 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Simple component |
| 22 | Trypticase peptone | 2 G_PER_L | Properties: Undefined component | |
| 23 |
Sodium formate
CHO2.Na 68.007 g/mol
|
0.25 G_PER_L |
CHEBI:62965
sodium formate
|
Properties: Defined component, Inorganic compound, Simple component |
| 24 |
Na2-EDTA·2H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
5 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 25 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 26 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.638338Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3253 |
| 2026-01-28T19:48:09.734753Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CO2; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-13T06:20:08.093610Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009685 |
| 2026-03-17T06:29:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.096808+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MSF medium" to "msf_medium" for consistent matching |
| 2026-05-16T09:04:26.968001+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:38.774656+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.247613+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |