Overview
- External ontology
- TOGO:M3287 — MNYP medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3287 Original source: NBRC - NBRC_M1647-2 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1647
Composition
flowchart LR
CultureMech_009701["mnyp_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009701 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Sodium_acetate["Sodium acetate"]
CultureMech_009701 --> ing1_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing1_Sodium_acetate -.-> CHEBI_32954
ing2_Mannitol["Mannitol"]
CultureMech_009701 --> ing2_Mannitol
CHEBI_29864["CHEBI:29864"]
ing2_Mannitol -.-> CHEBI_29864
ing3_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_009701 --> ing3_Bacto_Yeast_Extract__Difco_
ing4_Tween_80["Tween 80"]
CultureMech_009701 --> ing4_Tween_80
CHEBI_53426["CHEBI:53426"]
ing4_Tween_80 -.-> CHEBI_53426
ing5_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009701 --> ing5_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_7H2O -.-> CHEBI_31795
ing6_NaCl["NaCl"]
CultureMech_009701 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_009701 --> ing7_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_7H2O -.-> CHEBI_75836
ing8_MnSO4_4H2O["MnSO4·4H2O"]
CultureMech_009701 --> ing8_MnSO4_4H2O
CHEBI_86358["CHEBI:86358"]
ing8_MnSO4_4H2O -.-> CHEBI_86358
ing9_HCl["HCl"]
CultureMech_009701 --> ing9_HCl
CHEBI_17883["CHEBI:17883"]
ing9_HCl -.-> CHEBI_17883
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Collapsed 3 identical duplicates: 1.0, 1.0, 1.0] |
| 2 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
2 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Defined component, Simple component, Organic compound |
| 3 |
Mannitol
C6H14O6 182.172 g/mol
|
10 G_PER_L |
CHEBI:29864
mannitol
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 4 | Bacto Yeast Extract (Difco) | 10 G_PER_L | Properties: Undefined component | |
| 5 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
50 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Defined component, Organic compound, Simple component |
| 6 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
40 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
2 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 9 |
MnSO4·4H2O
4H2O.Mn.O4S 223.061 g/mol
|
2 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 10 |
HCl
HCl 36.461 g/mol
|
variable VARIABLE |
CHEBI:17883
hydrogen chloride
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution |
Solutions
Hipolypepton*
Dose: 5 G_PER_L
Properties: Undefined component
Tween 80 solution**
Dose: 10 G_PER_L
Salts solution***
Dose: 5 G_PER_L
See mediadive_4429_Salts_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.262445Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M3287 |
| 2026-01-28T19:48:30.585392Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: HCl |
| 2026-03-09T08:04:50.868650+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:22.168228Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:08.246070Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009701 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.233302+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MNYP medium" to "mnyp_medium" for consistent matching |
| 2026-05-16T09:03:17.165639+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:40.460303+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:29.768692+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-09-02T07:37:49.037331+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |