mbm_medium (CultureMech:009713)

Overview

External ontology

TOGO:M335 — MBM Medium

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M335 Original source: JCM - JCM_M340 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=340

Composition

flowchart LR
    CultureMech_009713["mbm_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009713 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_009713 --> ing1_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_2H2O -.-> CHEBI_86158
    ing2_KH2PO4["KH2PO4"]
    CultureMech_009713 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NH4Cl["NH4Cl"]
    CultureMech_009713 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_009713 --> ing4_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_6H2O -.-> CHEBI_86345
    ing5_KCl["KCl"]
    CultureMech_009713 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_NaHCO3["NaHCO3"]
    CultureMech_009713 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_NaNO3["NaNO3"]
    CultureMech_009713 --> ing7_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing7_NaNO3 -.-> CHEBI_63005
    ing8_O2["O2"]
    CultureMech_009713 --> ing8_O2
    ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009713 --> ing9_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing9_Carbon_dioxide_gas -.-> CHEBI_16526
    ing10_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009713 --> ing10_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing10_Nitrogen_gas -.-> CHEBI_17997
    ing11_FeSO4_7H2O["FeSO4・7H2O"]
    CultureMech_009713 --> ing11_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_7H2O -.-> CHEBI_75836
    ing12_EDTA["EDTA"]
    CultureMech_009713 --> ing12_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing12_EDTA -.-> CHEBI_4735

Ingredients (13)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	901.0 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 1.0, 900.0]
2	CaCl2・2H2O Ca.2Cl.2H2O 147.014 g/mol	0.1 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
3	KH2PO4 H2O4P.K 136.084 g/mol	0.2 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
4	NH4Cl Cl.H4N 53.492 g/mol	0.2 G_PER_L	CHEBI:31206 ammonium chloride	Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
5	MgCl2・6H2O 2Cl.6H2O.Mg 203.301 g/mol	0.4 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
6	KCl Cl.K 74.551 g/mol	0.2 G_PER_L	CHEBI:32588 potassium chloride	Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
7	NaHCO3 CHO3.Na 84.006 g/mol	1 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
8	NaNO3 NO3.Na 84.994 g/mol	0.2 G_PER_L	CHEBI:63005 sodium nitrate	Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
9	O2	variable VARIABLE		Properties: Gas
10	Carbon dioxide gas CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Properties: ガス
11	Nitrogen gas N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Properties: ガス
12	FeSO4・7H2O Fe.7H2O.O4S 278.013 g/mol	0.2 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
13	EDTA C10H16N2O8 292.244 g/mol	0.5 G_PER_L	CHEBI:4735 ethylenediaminetetraacetic acid	Properties: Defined component, Organic compound, Simple component

Solutions

SL--4 trace element solution (see below)

Dose: 2 G_PER_L

Trace vitamins (see Medium [M190])

Dose: variable VARIABLE

Cross-reference to Medium M190

5% Na2S·9H2O solution

Dose: 10 G_PER_L

Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound

SL-6 trace element solution (see Medium [M158])

Dose: 100 G_PER_L

Cross-reference to Medium M158

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:54.868669Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M335
2026-01-28T19:48:12.644874Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Trace vitamins (see Medium [M190]); Added default concentration for ingredient: O2; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:20.894880+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 4 solution(s) from ingredients
2026-03-13T03:08:48.929254Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 1 duplicate ingredient(s)
2026-03-13T06:20:08.319019Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009713
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:52.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:27.309470+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MBM Medium" to "mbm_medium" for consistent matching
2026-05-16T09:03:17.217775+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:40.513176+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:50.347947+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.