Overview
- External ontology
- TOGO:M33 — Marine Broth 2216
- Applications
-
- Microbial cultivation
- Notes
- TOGO M33 mirrors JCM Medium 41, which lists 37.4 g Marine broth 2216 (BD-Difco) in 1.0 L distilled water; ATCC Medium 2 confirms the same 37.4 g Marine Broth 2216 product and prints the scratch Difco Marine Agar/Broth 2216 formulation with agar omitted.
Composition
flowchart LR
CultureMech_009718["marine_broth_2216"]
ing0_Peptone["Peptone"]
CultureMech_009718 --> ing0_Peptone
ing1_Yeast_Extract["Yeast Extract"]
CultureMech_009718 --> ing1_Yeast_Extract
ing2_Ferric_Citrate["Ferric Citrate"]
CultureMech_009718 --> ing2_Ferric_Citrate
CHEBI_144421["CHEBI:144421"]
ing2_Ferric_Citrate -.-> CHEBI_144421
ing3_Sodium_Chloride["Sodium Chloride"]
CultureMech_009718 --> ing3_Sodium_Chloride
CHEBI_26710["CHEBI:26710"]
ing3_Sodium_Chloride -.-> CHEBI_26710
ing4_Magnesium_Chloride["Magnesium Chloride"]
CultureMech_009718 --> ing4_Magnesium_Chloride
CHEBI_6636["CHEBI:6636"]
ing4_Magnesium_Chloride -.-> CHEBI_6636
ing5_Sodium_Sulfate["Sodium Sulfate"]
CultureMech_009718 --> ing5_Sodium_Sulfate
CHEBI_32149["CHEBI:32149"]
ing5_Sodium_Sulfate -.-> CHEBI_32149
ing6_Calcium_Chloride["Calcium Chloride"]
CultureMech_009718 --> ing6_Calcium_Chloride
CHEBI_3312["CHEBI:3312"]
ing6_Calcium_Chloride -.-> CHEBI_3312
ing7_Potassium_Chloride["Potassium Chloride"]
CultureMech_009718 --> ing7_Potassium_Chloride
CHEBI_32588["CHEBI:32588"]
ing7_Potassium_Chloride -.-> CHEBI_32588
ing8_Sodium_Bicarbonate["Sodium Bicarbonate"]
CultureMech_009718 --> ing8_Sodium_Bicarbonate
CHEBI_32139["CHEBI:32139"]
ing8_Sodium_Bicarbonate -.-> CHEBI_32139
ing9_Potassium_Bromide["Potassium Bromide"]
CultureMech_009718 --> ing9_Potassium_Bromide
CHEBI_32030["CHEBI:32030"]
ing9_Potassium_Bromide -.-> CHEBI_32030
ing10_Strontium_Chloride["Strontium Chloride"]
CultureMech_009718 --> ing10_Strontium_Chloride
CHEBI_36383["CHEBI:36383"]
ing10_Strontium_Chloride -.-> CHEBI_36383
ing11_Boric_Acid["Boric Acid"]
CultureMech_009718 --> ing11_Boric_Acid
CHEBI_33118["CHEBI:33118"]
ing11_Boric_Acid -.-> CHEBI_33118
ing12_Sodium_Silicate["Sodium Silicate"]
CultureMech_009718 --> ing12_Sodium_Silicate
CHEBI_60720["CHEBI:60720"]
ing12_Sodium_Silicate -.-> CHEBI_60720
ing13_Sodium_Fluoride["Sodium Fluoride"]
CultureMech_009718 --> ing13_Sodium_Fluoride
CHEBI_28741["CHEBI:28741"]
ing13_Sodium_Fluoride -.-> CHEBI_28741
ing14_Ammonium_Nitrate["Ammonium Nitrate"]
CultureMech_009718 --> ing14_Ammonium_Nitrate
CHEBI_63038["CHEBI:63038"]
ing14_Ammonium_Nitrate -.-> CHEBI_63038
ing15_Disodium_Phosphate["Disodium Phosphate"]
CultureMech_009718 --> ing15_Disodium_Phosphate
CHEBI_34683["CHEBI:34683"]
ing15_Disodium_Phosphate -.-> CHEBI_34683
ing16_Distilled_water["Distilled water"]
CultureMech_009718 --> ing16_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing16_Distilled_water -.-> CHEBI_15377
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 5.0 G_PER_L |
MICRO:0000178 (source link unavailable)
peptone
|
ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation. |
| 2 | Yeast Extract | 1.0 G_PER_L |
FOODON:03315426
yeast extract
|
ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation. |
| 3 |
Ferric Citrate
C6H5FeO7 244.944 g/mol
|
0.1 G_PER_L |
CHEBI:144421
iron(III) citrate
|
ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation. |
| 4 |
Sodium Chloride
Cl.Na 58.443 g/mol
|
19.45 G_PER_L |
CHEBI:26710
sodium chloride
|
ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation. |
| 5 |
Magnesium Chloride
2Cl.Mg 95.211 g/mol
|
8.8 G_PER_L |
CHEBI:6636
magnesium dichloride
|
ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation. |
| 6 |
Sodium Sulfate
2Na.O4S 142.043 g/mol
|
3.24 G_PER_L |
CHEBI:32149
sodium sulfate
|
ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation. |
| 7 |
Calcium Chloride
Ca.2Cl 110.984 g/mol
|
1.8 G_PER_L |
CHEBI:3312
calcium dichloride
|
ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation. |
| 8 |
Potassium Chloride
Cl.K 74.551 g/mol
|
0.55 G_PER_L |
CHEBI:32588
potassium chloride
|
ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation. |
| 9 |
Sodium Bicarbonate
CHO3.Na 84.006 g/mol
|
0.16 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation. |
| 10 |
Potassium Bromide
Br.K 119.002 g/mol
|
0.08 G_PER_L |
CHEBI:32030
potassium bromide
|
ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation. |
| 11 |
Strontium Chloride
2Cl.Sr 158.526 g/mol
|
0.034 G_PER_L |
CHEBI:36383
strontium dichloride
|
ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation. |
| 12 |
Boric Acid
H3BO3 61.833 g/mol
|
0.022 G_PER_L |
CHEBI:33118
boric acid
|
ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation. |
| 13 |
Sodium Silicate
2Na.O3Si 122.063 g/mol
|
0.004 G_PER_L |
CHEBI:60720
sodium silicate
|
ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation. |
| 14 |
Sodium Fluoride
F.Na 41.988 g/mol
|
0.0024 G_PER_L |
CHEBI:28741
sodium fluoride
|
ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation. |
| 15 |
Ammonium Nitrate
H4N.NO3 80.043 g/mol
|
0.0016 G_PER_L |
CHEBI:63038
ammonium nitrate
|
ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation. |
| 16 |
Disodium Phosphate
HO4P.2Na 141.958 g/mol
|
0.008 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
ATCC Medium 2 prints this component in the Difco Marine Agar/Broth 2216 scratch formulation. |
| 17 |
Distilled water
H2O 18.015 g/mol
|
1000.0 ML_PER_L |
CHEBI:15377
water
|
ATCC Medium 2 prints 1000.0 mL distilled water in the scratch formulation. |
Preparation
- Prepare Marine Broth 2216 from the Difco Marine Agar/Broth 2216 scratch formulation in 1000 mL distilled water, omitting agar.
- Autoclave at 121 C.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.862096Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M33 |
| 2026-01-28T19:48:20.540950Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:08.344209Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009718 |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.334521+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Marine Broth 2216" to "marine_broth_2216" for consistent matching |
| 2026-06-05T07:40:40.546565+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-06T00:00:00-07:00 | repair_atcc_score40.py | LINKED_ATCC_SCORE40_DUPLICATES | Added or refreshed bacterial/TOGO_M2481_Marine_Broth_2216.yaml as a child of bacterial/TOGO_M33_Marine_Broth_2216.yaml. |
| 2026-09-10T00:00:00-07:00 | repair_togo_commercial_score20.py | RESOLVED_TOGO_COMMERCIAL_SCORE20_GRAPH | TOGO M33 mirrors JCM Medium 41, which lists 37.4 g Marine broth 2216 (BD-Difco) in 1.0 L distilled water; ATCC Medium 2 confirms the same 37.4 g Marine Broth 2216 product and prints the scratch Difco Marine Agar/Broth 2216 formulation with agar omitted. |
| 2026-09-12T00:00:00-07:00 | repair_togo_m2255_half_marine_score15.py | LINKED_TOGO_M2255_HALF_MARINE_VARIANT | Linked TOGO M2255 / ATCC Medium 2515 as a half-strength Marine Broth 2216 concentration variant with optional agar. |