Overview
- External ontology
- TOGO:M353 — 1% Tryptone Agar
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- TOGO M353 imports JCM Medium 359 as 1% Tryptone Agar. JCM Medium 359 lists 10.0 g Tryptone (BD-Difco), 15.0 g Bacto agar (BD-Difco), 1.0 L Distilled water, and pH 7.0. JCM states to autoclave at 121 C for 15 min unless otherwise stated.
Composition
flowchart LR
CultureMech_009733["1_tryptone_agar"]
ing0_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_009733 --> ing0_Tryptone__BD_Difco_
ing1_Bacto_agar__BD_Difco_["Bacto agar (BD-Difco)"]
CultureMech_009733 --> ing1_Bacto_agar__BD_Difco_
CHEBI_2509["CHEBI:2509"]
ing1_Bacto_agar__BD_Difco_ -.-> CHEBI_2509
ing2_Distilled_water["Distilled water"]
CultureMech_009733 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone (BD-Difco) | 10.0 G_PER_L | JCM Medium 359 lists the BD-Difco tryptone product; the product is source-disclosed but not reducible to one ChEBI molecule. | |
| 2 | Bacto agar (BD-Difco) | 15.0 G_PER_L |
CHEBI:2509
agar
|
JCM Medium 359 lists 15.0 g/L Bacto agar from BD-Difco. |
| 3 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
JCM Medium 359 lists 1.0 L Distilled water. |
Preparation
- Adjust pH to 7.0.
- Autoclave at 121 C for 15 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.896754Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M353 |
| 2026-01-28T19:48:38.858536Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:08.444077Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009733 |
| 2026-03-17T06:30:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.431499+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "1% Tryptone Agar" to "1_tryptone_agar" for consistent matching |
| 2026-06-05T07:40:40.650351+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m353_score15.py | RESOLVED_TOGO_M353_SCORE15 | TOGO M353 imports JCM Medium 359 as 1% Tryptone Agar. JCM Medium 359 lists 10.0 g Tryptone (BD-Difco), 15.0 g Bacto agar (BD-Difco), 1.0 L Distilled water, and pH 7.0. JCM states to autoclave at 121 C for 15 min unless otherwise stated. Corrected the imported water unit artifact, added JCM autoclave metadata, kept Tryptone (BD-Difco) intentionally unmapped, and linked the TOGO import to JCM Medium 359. |