py4sr_agar

CultureMech:009738 bacterial COMPLEX SOLID_AGAR
Original name: PY4SR Agar

Overview

External ontology
TOGO:M358 — PY4SR Agar
Applications
  • Microbial cultivation
pH
6.7
Notes
TOGO M358 imports JCM Medium 364 PY4SR Agar. JCM Medium 364 uses JCM Medium 363 PY4S Agar supplemented with 50 ml/L plant residue extract prepared by autoclaving rice straw in water at a 1:5 ratio for 20 min at 120 C and using the supernatant after centrifugation.

Ingredients (0)

No ingredients recorded.

Preparation

  1. Autoclave rice straw in water at a 1:5 ratio at 120 C for 20 min to prepare Plant residue extract.
  2. After centrifugation, use the supernatant to supplement PY4S Agar with 50 ml/L Plant residue extract.

Solutions

Plant residue extract

Dose: 50.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Rice straw variable VARIABLE JCM Medium 364 prepares Plant residue extract by autoclaving rice straw in water at a 1:5 ratio; the final soluble rice-straw concentration is not specified.
Distilled water variable VARIABLE JCM Medium 364 prepares Plant residue extract by autoclaving rice straw in water at a 1:5 ratio.
JCM Medium 364 adds 50 ml/L Plant residue extract to PY4S Agar.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.906326Z togo-import Imported from TOGO Medium Source: JCM, ID: M358
2026-01-28T19:48:25.210017Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: water; Added default concentration for ingredient: rice straw
2026-03-09T08:04:41.687951+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:08.490339Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009738
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:18.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:27.477613+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PY4SR Agar" to "py4sr_agar" for consistent matching
2026-05-16T09:03:17.389523+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:40.701560+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m358_score15.py RESOLVED_TOGO_M358_SCORE15 TOGO M358 imports JCM Medium 364 PY4SR Agar. JCM Medium 364 uses JCM Medium 363 PY4S Agar supplemented with 50 ml/L plant residue extract prepared by autoclaving rice straw in water at a 1:5 ratio for 20 min at 120 C and using the supernatant after centrifugation. Corrected the imported stock-reference unit artifact, represented the plant residue extract as a 50 ml/L supplement, and linked the record to JCM Medium 363 PY4S Agar.