Overview
- External ontology
- TOGO:M373 — Alkaliphilus Crotonatoxidans Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M373 Original source: JCM - JCM_M378 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=378
Composition
flowchart LR
CultureMech_009754["alkaliphilus_crotonatoxidans_medium"]
ing0_Sol_1["Sol.1"]
CultureMech_009754 --> ing0_Sol_1
ing1_Distilled_water["Distilled water"]
CultureMech_009754 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_NaCl["NaCl"]
CultureMech_009754 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009754 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009754 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_009754 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_Resazurin["Resazurin"]
CultureMech_009754 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009754 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_NaHCO3["NaHCO3"]
CultureMech_009754 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009754 --> ing9_Yeast_extract__BD_Difco_
ing10_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_009754 --> ing10_Tryptone__BD_Difco_
ing11_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009754 --> ing11_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing11_Carbon_dioxide_gas -.-> CHEBI_16526
ing12_N2["N2"]
CultureMech_009754 --> ing12_N2
CHEBI_17997["CHEBI:17997"]
ing12_N2 -.-> CHEBI_17997
ing13_Crotonic_acid["Crotonic acid"]
CultureMech_009754 --> ing13_Crotonic_acid
CHEBI_41131["CHEBI:41131"]
ing13_Crotonic_acid -.-> CHEBI_41131
ing14_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_009754 --> ing14_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_2H2O -.-> CHEBI_75213
ing15_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_009754 --> ing15_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing15_CoCl2_6H2O -.-> CHEBI_35696
ing16_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_009754 --> ing16_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_6H2O -.-> CHEBI_34887
ing17_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_009754 --> ing17_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4_7H2O -.-> CHEBI_32312
ing18_CuCl2_2H2O["CuCl2・2H2O"]
CultureMech_009754 --> ing18_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_2H2O -.-> CHEBI_86318
ing19_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009754 --> ing19_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing19_Nitrilotriacetic_acid -.-> CHEBI_44557
ing20_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_009754 --> ing20_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing20_MnSO4_xH2O -.-> CHEBI_86360
ing21_Fe_NH4_2_SO4_2_6H2O["Fe(NH4)2(SO4)2・6H2O"]
CultureMech_009754 --> ing21_Fe_NH4_2_SO4_2_6H2O
CHEBI_76181["CHEBI:76181"]
ing21_Fe_NH4_2_SO4_2_6H2O -.-> CHEBI_76181
ing22_Na2WO4["Na2WO4"]
CultureMech_009754 --> ing22_Na2WO4
CHEBI_63940["CHEBI:63940"]
ing22_Na2WO4 -.-> CHEBI_63940
ing23_Na2SeO4["Na2SeO4"]
CultureMech_009754 --> ing23_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing23_Na2SeO4 -.-> CHEBI_77775
ing24_KOH["KOH"]
CultureMech_009754 --> ing24_KOH
CHEBI_32035["CHEBI:32035"]
ing24_KOH -.-> CHEBI_32035
ing25_L__Cysteine_HCL_H2O["L--Cysteine・HCL・H2O"]
CultureMech_009754 --> ing25_L__Cysteine_HCL_H2O
CHEBI_91248["CHEBI:91248"]
ing25_L__Cysteine_HCL_H2O -.-> CHEBI_91248
ing26_Na2S_9H2O["Na2S・9H2O"]
CultureMech_009754 --> ing26_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing26_Na2S_9H2O -.-> CHEBI_76209
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sol.1 | 900 G_PER_L | ||
| 2 |
Distilled water
H2O 18.015 g/mol
|
1021.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 5 duplicates: 900.0, 100.0, 1.0, 10.0, 10.0] |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.4 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.4 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 8 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.33 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
3.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 | Yeast extract (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 11 | Tryptone (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 12 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 13 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 14 |
Crotonic acid
C4H6O2 86.09 g/mol
|
0.86 G_PER_L |
CHEBI:41131
crotonic acid
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 15 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.02 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
0.2 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.2 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
CuCl2・2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.02 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
2 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 21 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
1 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Fe(NH4)2(SO4)2・6H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.8 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
Na2WO4
2Na.O4W 293.816 g/mol
|
0.02 G_PER_L |
CHEBI:63940
sodium tungstate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.02 G_PER_L |
CHEBI:77775
sodium selenate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 26 |
L--Cysteine・HCL・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 27 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
Solutions
crotonic acid solution
Dose: 100 G_PER_L
cysteine・HCl solution
Dose: 10 G_PER_L
Na2S・9H2O solution
Dose: 10 G_PER_L
Trace metal solution (see below)
Dose: 10 G_PER_L
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.934292Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M373 |
| 2026-01-28T19:48:38.648769Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: KOH |
| 2026-03-09T08:05:04.714244+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:09:36.679783Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:20:08.622383Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009754 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.615761+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Alkaliphilus Crotonatoxidans Medium" to "alkaliphilus_crotonatoxidans_medium" for consistent matching |
| 2026-05-16T09:03:17.507246+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:40.832171+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:34.153160+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:46:50.446432+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |