carnobacterium_pleistocaenium_medium

CultureMech:009755 bacterial COMPLEX LIQUID
Original name: Carnobacterium Pleistocaenium Medium

Overview

External ontology
TOGO:M374 — Carnobacterium Pleistocaenium Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M374 Original source: JCM - JCM_M379 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=379

Composition

flowchart LR
    CultureMech_009755["carnobacterium_pleistocaenium_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009755 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_009755 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_009755 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KH2PO4["KH2PO4"]
    CultureMech_009755 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_009755 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_D__Glucose["D--Glucose"]
    CultureMech_009755 --> ing5_D__Glucose
    ing6_Resazurin["Resazurin"]
    CultureMech_009755 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_009755 --> ing7_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_9H2O -.-> CHEBI_76209
    ing8_KCl["KCl"]
    CultureMech_009755 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588
    ing9_NaHCO3["NaHCO3"]
    CultureMech_009755 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_CaSO4_2H2O["CaSO4・2H2O"]
    CultureMech_009755 --> ing10_CaSO4_2H2O
    CHEBI_32583["CHEBI:32583"]
    ing10_CaSO4_2H2O -.-> CHEBI_32583
    ing11_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_009755 --> ing11_Yeast_extract__BD_Difco_
    ing12_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
    CultureMech_009755 --> ing12_Bacto_peptone__BD_Difco_
    ing13_N2["N2"]
    CultureMech_009755 --> ing13_N2
    CHEBI_17997["CHEBI:17997"]
    ing13_N2 -.-> CHEBI_17997

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
4 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
5 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
6 D--Glucose 5 G_PER_L Role: Carbon source; Properties: Defined component, Simple component, Organic compound
7 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component
8 Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
0.4 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Defined component, Simple component, Inorganic compound
9 KCl
Cl.K 74.551 g/mol
0.3 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
10 NaHCO3
CHO3.Na 84.006 g/mol
0.4 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
11 CaSO4・2H2O
Ca.2H2O.O4S 172.171 g/mol
12.5 G_PER_L CHEBI:32583
calcium sulfate dihydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
12 Yeast extract (BD-Difco) 0.2 G_PER_L Properties: Undefined component
13 Bacto peptone (BD-Difco) 1 G_PER_L Properties: Undefined component
14 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas

Solutions

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Trace minerals solution (see Medium [M221])

Dose: 1 G_PER_L

Cross-reference to Medium M221

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.937968Z togo-import Imported from TOGO Medium Source: JCM, ID: M374
2026-01-28T19:48:31.764593Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2
2026-03-09T08:04:52.908056+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:20:08.633573Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009755
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:33.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:27.626643+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Carnobacterium Pleistocaenium Medium" to "carnobacterium_pleistocaenium_medium" for consistent matching
2026-05-16T09:03:17.520266+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:40.844848+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:30.103714+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:50.453467+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.