Overview
- External ontology
- TOGO:M382 — PYGV Agar
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- JCM Medium 387 PYGV Agar lists, per liter, 0.25 g Peptone, 0.25 g Yeast extract, 20 ml Mineral salt solution from JCM Medium 304, 10 ml 2.5% Glucose solution, 10 ml Vitamin solution from JCM Medium 304, 15 g Agar, and 960 ml Distilled water. The final pH is 7.5. The base medium is mixed, gently heated to a boil, autoclaved, cooled to 45-50 degrees C, supplemented aseptically with filter-sterilized glucose and vitamin solutions, and adjusted to pH 7.5 with sterile KOH if necessary.
Composition
flowchart LR
CultureMech_009763["pygv_agar"]
ing0_Peptone["Peptone"]
CultureMech_009763 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_009763 --> ing1_Yeast_extract
ing2_Agar["Agar"]
CultureMech_009763 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_Distilled_water["Distilled water"]
CultureMech_009763 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
ing4_KOH["KOH"]
CultureMech_009763 --> ing4_KOH
CHEBI_32035["CHEBI:32035"]
ing4_KOH -.-> CHEBI_32035
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 0.25 G_PER_L | TOGO M382 / JCM Medium 387 lists 0.25 g/L Peptone. | |
| 2 | Yeast extract | 0.25 G_PER_L | TOGO M382 / JCM Medium 387 lists 0.25 g/L Yeast extract. | |
| 3 | Agar | 15.0 G_PER_L |
CHEBI:2509
agar
|
TOGO M382 / JCM Medium 387 lists 15.0 g/L Agar. |
| 4 |
Distilled water
H2O 18.015 g/mol
|
960.0 ML_PER_L |
CHEBI:15377
water
|
TOGO M382 / JCM Medium 387 lists 960.0 ml/L Distilled water. |
| 5 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
JCM Medium 387 adjusts the medium to pH 7.5 with sterile KOH, if necessary. |
Preparation
- Mix peptone, yeast extract, mineral salt solution, agar, and distilled water.
- Gently heat and bring to a boil.
- Autoclave at 121 degrees C for 15 min.
- Cool to 45-50 degrees C.
- Aseptically add the filter-sterilized glucose solution and vitamin solution.
- Adjust to pH 7.5 with sterile KOH, if necessary.
Solutions
Mineral salt solution
Dose: 20.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgSO4 x 7H2O | 29.7 G_PER_L | TOGO M299 / JCM Medium 304 lists 29.7 g/L MgSO4 x 7H2O. |
| Nitrilotriacetic acid | 10.0 G_PER_L | TOGO M299 / JCM Medium 304 lists 10.0 g/L Nitrilotriacetic acid. |
| CaCl2 x 2H2O | 3.34 G_PER_L | TOGO M299 / JCM Medium 304 lists 3.34 g/L CaCl2 x 2H2O. |
| FeSO4 x 7H2O | 99.0 MG_PER_L | TOGO M299 / JCM Medium 304 lists 99.0 mg/L FeSO4 x 7H2O. |
| Na2MoO4 x 2H2O | 13.0 MG_PER_L | TOGO M299 / JCM Medium 304 lists 13.0 mg/L Na2MoO4 x 2H2O. |
| Metals 44 | 50.0 ML_PER_L | JCM Medium 304 adds 50.0 ml/L Metals 44 from JCM Medium 149. |
| Distilled water | 950.0 ML_PER_L | TOGO M299 / JCM Medium 304 lists 950.0 ml/L Distilled water. |
TOGO M382 / JCM Medium 387 adds 20.0 ml/L Mineral salt solution.
2.5% Glucose solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Glucose | 2.5 PERCENT_W_V | TOGO M382 / JCM Medium 387 lists 2.5 % w/v Glucose. |
TOGO M382 / JCM Medium 387 adds 10.0 ml/L 2.5% Glucose solution.
Vitamin solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 2.0 MG_PER_L | TOGO M299 / JCM Medium 304 lists 2.0 mg/L Biotin. |
| Folic acid | 2.0 MG_PER_L | TOGO M299 / JCM Medium 304 lists 2.0 mg/L Folic acid. |
| Pyridoxine HCl | 10.0 MG_PER_L | TOGO M299 / JCM Medium 304 lists 10.0 mg/L Pyridoxine HCl. |
| Riboflavin | 5.0 MG_PER_L | TOGO M299 / JCM Medium 304 lists 5.0 mg/L Riboflavin. |
| Thiamine HCl | 5.0 MG_PER_L | TOGO M299 / JCM Medium 304 lists 5.0 mg/L Thiamine HCl. |
| Nicotinamide | 5.0 MG_PER_L | TOGO M299 / JCM Medium 304 lists 5.0 mg/L Nicotinamide. |
| Calcium pantothenate | 5.0 MG_PER_L | TOGO M299 / JCM Medium 304 lists 5.0 mg/L Calcium pantothenate. |
| Vitamin B12 | 0.1 MG_PER_L | TOGO M299 / JCM Medium 304 lists 0.1 mg/L Vitamin B12. |
| p-Aminobenzoic acid | 5.0 MG_PER_L | TOGO M299 / JCM Medium 304 lists 5.0 mg/L p-Aminobenzoic acid. |
| Distilled water | 1.0 L | TOGO M299 / JCM Medium 304 lists 1.0 L Distilled water. |
TOGO M382 / JCM Medium 387 adds 10.0 ml/L Vitamin solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.951799Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M382 |
| 2026-01-28T19:48:24.283447Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH |
| 2026-03-09T08:04:40.140992+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:20:08.692954Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009763 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:16.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.688334+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYGV Agar" to "pygv_agar" for consistent matching |
| 2026-05-16T09:03:17.580972+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:40.914115+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m382_score15.py | RESOLVED_TOGO_M382_SCORE15 | JCM Medium 387 PYGV Agar lists, per liter, 0.25 g Peptone, 0.25 g Yeast extract, 20 ml Mineral salt solution from JCM Medium 304, 10 ml 2.5% Glucose solution, 10 ml Vitamin solution from JCM Medium 304, 15 g Agar, and 960 ml Distilled water. The final pH is 7.5. The base medium is mixed, gently heated to a boil, autoclaved, cooled to 45-50 degrees C, supplemented aseptically with filter-sterilized glucose and vitamin solutions, and adjusted to pH 7.5 with sterile KOH if necessary. |