desulfovibrio_medium

Overview

External ontology

TOGO:M384 — Desulfovibrio Medium

Applications

• Microbial cultivation

pH range

{'min': 6.8, 'max': 7.0, 'notes': 'JCM Medium 389 says adjust pH to 6.8-7.0, if necessary.'}

Notes

TOGO M384 mirrors JCM Medium 389: Desulfovibrio Medium uses sulfate, sodium lactate, yeast extract, reducing agents, resazurin, and anoxic N2 handling.

Composition

flowchart LR
    CultureMech_009765["desulfovibrio_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_009765 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_NH4Cl["NH4Cl"]
    CultureMech_009765 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_Na2SO4["Na2SO4"]
    CultureMech_009765 --> ing2_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing2_Na2SO4 -.-> CHEBI_32149
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_009765 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_009765 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Sodium_lactate["Sodium lactate"]
    CultureMech_009765 --> ing5_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing5_Sodium_lactate -.-> CHEBI_75228
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_009765 --> ing6_Yeast_extract
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_009765 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_Sodium_thioglycolate["Sodium thioglycolate"]
    CultureMech_009765 --> ing8_Sodium_thioglycolate
    CHEBI_86481["CHEBI:86481"]
    ing8_Sodium_thioglycolate -.-> CHEBI_86481
    ing9_Ascorbic_acid["Ascorbic acid"]
    CultureMech_009765 --> ing9_Ascorbic_acid
    CHEBI_22652["CHEBI:22652"]
    ing9_Ascorbic_acid -.-> CHEBI_22652
    ing10_Resazurin["Resazurin"]
    CultureMech_009765 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_Distilled_water["Distilled water"]
    CultureMech_009765 --> ing11_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing11_Distilled_water -.-> CHEBI_15377

Ingredients (12)

Preparation

1. Mix ingredients except FeSO4 x 7 H2O, sodium thioglycolate, and ascorbic acid.
2. Bring the medium to a boil and cool to room temperature under an N2 gas stream.
3. Add FeSO4 x 7 H2O in 10 mL distilled water plus sodium thioglycolate and ascorbic acid in 10 mL distilled water.
4. Adjust pH to 7.8 with NaOH; adjust to pH 6.8-7.0 if necessary.
5. While swirling under an N2 gas stream, dispense into culture vessels and seal with butyl rubber stoppers.
6. Autoclave the sealed medium.

Target organisms

No target organisms are recorded.

Provenance