desulfobacterium_cetonicum_medium (CultureMech:009773)

Overview

External ontology

TOGO:M391 — Desulfobacterium Cetonicum Medium

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M391 Original source: JCM - JCM_M395 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=395

Composition

flowchart LR
    CultureMech_009773["desulfobacterium_cetonicum_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009773 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_009773 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_009773 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_009773 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_009773 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Resazurin["Resazurin"]
    CultureMech_009773 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_009773 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_009773 --> ing7_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_9H2O -.-> CHEBI_76209
    ing8_NaHCO3["NaHCO3"]
    CultureMech_009773 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_Na2SO4["Na2SO4"]
    CultureMech_009773 --> ing9_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing9_Na2SO4 -.-> CHEBI_32149
    ing10_Sodium_butyrate["Sodium butyrate"]
    CultureMech_009773 --> ing10_Sodium_butyrate
    CHEBI_64103["CHEBI:64103"]
    ing10_Sodium_butyrate -.-> CHEBI_64103
    ing11_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009773 --> ing11_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing11_Carbon_dioxide_gas -.-> CHEBI_16526
    ing12_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009773 --> ing12_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing12_Nitrogen_gas -.-> CHEBI_17997

Ingredients (13)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2	NaCl Cl.Na 58.443 g/mol	10 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3	CaCl2・2H2O Ca.2Cl.2H2O 147.014 g/mol	0.05 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
4	KH2PO4 H2O4P.K 136.084 g/mol	0.7 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5	NH4Cl Cl.H4N 53.492 g/mol	0.3 G_PER_L	CHEBI:31206 ammonium chloride	Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component
6	Resazurin C12H7NO4 229.191 g/mol	1 G_PER_L	CHEBI:8806 Resazurin	Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component
7	MgCl2・6H2O 2Cl.6H2O.Mg 203.301 g/mol	1.7 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
8	Na2S・9H2O 9H2O.2Na.S 240.182 g/mol	0.3 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Role: Reducing agent; Properties: Defined component, Simple component, Inorganic compound
9	NaHCO3 CHO3.Na 84.006 g/mol	2 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
10	Na2SO4 2Na.O4S 142.043 g/mol	2.8 G_PER_L	CHEBI:32149 sodium sulfate	Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
11	Sodium butyrate C4H7O2.Na 110.088 g/mol	1.2 G_PER_L	CHEBI:64103 sodium butyrate	Properties: Defined component, Organic compound, Simple component
12	Carbon dioxide gas CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Properties: ガス
13	Nitrogen gas N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Properties: ガス

Solutions

FeCl2 solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Trace element solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:54.969766Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M391
2026-01-28T19:48:38.144268Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:05:03.794182+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 2 solution(s) from ingredients
2026-03-13T06:20:08.781856Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009773
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name
2026-03-17T06:30:46.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:27.777558+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Desulfobacterium Cetonicum Medium" to "desulfobacterium_cetonicum_medium" for consistent matching
2026-05-16T09:03:17.662304+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:41.000006+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:50.509629+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.