Overview
- External ontology
- TOGO:M399 — Halococcus Dombowskii Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M399 Original source: JCM - JCM_M402-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=402
Composition
flowchart LR
CultureMech_009781["halococcus_dombowskii_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009781 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_009781 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_009781 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009781 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009781 --> ing4_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_6H2O -.-> CHEBI_86345
ing5_KCl["KCl"]
CultureMech_009781 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Tris_base["Tris base"]
CultureMech_009781 --> ing6_Tris_base
CHEBI_9754["CHEBI:9754"]
ing6_Tris_base -.-> CHEBI_9754
ing7_agar["agar"]
CultureMech_009781 --> ing7_agar
CHEBI_2509["CHEBI:2509"]
ing7_agar -.-> CHEBI_2509
ing8_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_009781 --> ing8_Casamino_acids__BD_Difco_
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 2 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
200 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
20 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 7 |
Tris base
C4H11NO3 121.136 g/mol
|
12.1 G_PER_L |
CHEBI:9754
tris
|
Role: Buffer; Properties: Organic compound, Simple component, Defined component |
| 8 | agar | 20 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 9 | Casamino acids (BD-Difco) | 5 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.985069Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M399 |
| 2026-01-28T19:48:37.942539Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:08.847295Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009781 |
| 2026-03-17T06:30:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.840954+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Halococcus Dombowskii Medium" to "halococcus_dombowskii_medium" for consistent matching |
| 2026-06-05T07:40:41.070342+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |