nutrient_agar_with_50_soil_extract_and_5_nacl

CultureMech:009789 bacterial COMPLEX SOLID_AGAR
Original name: Nutrient Agar With 50% Soil Extract And 5% NaCl

Overview

External ontology
TOGO:M405 — Nutrient Agar With 50% Soil Extract And 5% NaCl
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M405 Original source: JCM - JCM_M407 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=407

Composition

flowchart LR
    CultureMech_009789["nutrient_agar_with_50_soil_extract_and_5_nacl"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009789 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_009789 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_Agar["Agar"]
    CultureMech_009789 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_Beef_extract["Beef extract"]
    CultureMech_009789 --> ing3_Beef_extract
    ing4_Peptone["Peptone"]
    CultureMech_009789 --> ing4_Peptone

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
500 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 NaCl
Cl.Na 58.443 g/mol
50 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
3 Agar 15 G_PER_L CHEBI:2509
agar
Role: Solidifying component
4 Beef extract 3 G_PER_L Properties: Undefined component
5 Peptone 5 G_PER_L

Solutions

Soil extract (see Medium [M94])

Dose: 500 G_PER_L

Cross-reference to Medium M94

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.997015Z togo-import Imported from TOGO Medium Source: JCM, ID: M405
2026-01-28T19:48:16.869124Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:27.869052+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:08.895471Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009789
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:01.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:27.887472+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Nutrient Agar With 50% Soil Extract And 5% NaCl" to "nutrient_agar_with_50_soil_extract_and_5_nacl" for consistent matching
2026-05-16T09:03:17.773607+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:41.120743+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.