Overview
- External ontology
- TOGO:M409 — Fe(III)-Reducer Marine Enrichment Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M409 Original source: JCM - JCM_M411 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=411
Composition
flowchart LR
CultureMech_009793["fe_iii_reducer_marine_enrichment_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009793 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009793 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_009793 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009793 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009793 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009793 --> ing5_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_6H2O -.-> CHEBI_86345
ing6_KCl["KCl"]
CultureMech_009793 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_NaHCO3["NaHCO3"]
CultureMech_009793 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8__NH4_2SO4["(NH4)2SO4"]
CultureMech_009793 --> ing8__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing8__NH4_2SO4 -.-> CHEBI_62946
ing9_NaBr["NaBr"]
CultureMech_009793 --> ing9_NaBr
CHEBI_63004["CHEBI:63004"]
ing9_NaBr -.-> CHEBI_63004
ing10_SrCl2_6H2O["SrCl2・6H2O"]
CultureMech_009793 --> ing10_SrCl2_6H2O
CHEBI_36385["CHEBI:36385"]
ing10_SrCl2_6H2O -.-> CHEBI_36385
ing11_FeCl2_4H2O["FeCl2・4H2O"]
CultureMech_009793 --> ing11_FeCl2_4H2O
CHEBI_86249["CHEBI:86249"]
ing11_FeCl2_4H2O -.-> CHEBI_86249
ing12_Na2SeO4["Na2SeO4"]
CultureMech_009793 --> ing12_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing12_Na2SeO4 -.-> CHEBI_77775
ing13_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009793 --> ing13_Yeast_extract__BD_Difco_
ing14_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_009793 --> ing14_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing14_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing15_Poorly_crystalline_iron_III__oxide__see_below_["Poorly crystalline iron(III) oxide (see below)"]
CultureMech_009793 --> ing15_Poorly_crystalline_iron_III__oxide__see_below_
ing16_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009793 --> ing16_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing16_Carbon_dioxide_gas -.-> CHEBI_16526
ing17_N2["N2"]
CultureMech_009793 --> ing17_N2
CHEBI_17997["CHEBI:17997"]
ing17_N2 -.-> CHEBI_17997
ing18_Hydrogen_gas["Hydrogen gas"]
CultureMech_009793 --> ing18_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing18_Hydrogen_gas -.-> CHEBI_18276
ing19_FeCl3["FeCl3"]
CultureMech_009793 --> ing19_FeCl3
CHEBI_30808["CHEBI:30808"]
ing19_FeCl3 -.-> CHEBI_30808
ing20_NaOH["NaOH"]
CultureMech_009793 --> ing20_NaOH
CHEBI_32145["CHEBI:32145"]
ing20_NaOH -.-> CHEBI_32145
ing21_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_009793 --> ing21_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_2H2O -.-> CHEBI_75213
ing22_H3BO3["H3BO3"]
CultureMech_009793 --> ing22_H3BO3
CHEBI_33118["CHEBI:33118"]
ing22_H3BO3 -.-> CHEBI_33118
ing23_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_009793 --> ing23_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing23_FeSO4_7H2O -.-> CHEBI_75836
ing24_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_009793 --> ing24_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing24_NiCl2_6H2O -.-> CHEBI_34887
ing25_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_009793 --> ing25_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing25_ZnSO4_7H2O -.-> CHEBI_32312
ing26_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_009793 --> ing26_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing26_CuSO4_5H2O -.-> CHEBI_31440
ing27_CoSO4_7H2O["CoSO4・7H2O"]
CultureMech_009793 --> ing27_CoSO4_7H2O
CHEBI_91244["CHEBI:91244"]
ing27_CoSO4_7H2O -.-> CHEBI_91244
ing28_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009793 --> ing28_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing28_Nitrilotriacetic_acid -.-> CHEBI_44557
ing29_Na2WO4_2H2O["Na2WO4・2H2O"]
CultureMech_009793 --> ing29_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing29_Na2WO4_2H2O -.-> CHEBI_63939
CultureMech_009793 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 3 identical duplicates: 1.0, 1.0, 1.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.15 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.15, 3.0] |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
20.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 19.0, 1.0] |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.4 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.3, 0.1] |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.42 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
9 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
NaBr
Br.Na 102.894 g/mol
|
0.05 G_PER_L |
CHEBI:63004
sodium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
SrCl2・6H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.02 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
FeCl2・4H2O
Cl2Fe.4H2O 198.811 g/mol
|
0.26 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.02 G_PER_L |
CHEBI:77775
sodium selenate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 | Yeast extract (BD-Difco) | 0.1 G_PER_L | Properties: Undefined component | |
| 15 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.044 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 16 | Poorly crystalline iron(III) oxide (see below) | 100 MILLIMOLAR | ||
| 17 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 18 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 19 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 20 |
FeCl3
Cl3Fe 162.204 g/mol
|
108 G_PER_L |
CHEBI:30808
iron trichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 22 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.025 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
CoSO4・7H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 30 |
Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.025 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 31 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 32 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Trace mineral solution (see below)
Dose: 10 G_PER_L
KOH solution
Dose: variable VARIABLE
Role: Mineral source; Properties: Defined component, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.001100Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M409 |
| 2026-01-28T19:48:29.581633Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: NaOH; Added default concentration for ingredient: KOH solution |
| 2026-03-09T08:04:49.142651+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:20.272669Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:20:08.921224Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009793 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:28.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.911974+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Fe(III)-Reducer Marine Enrichment Medium" to "fe_iii_reducer_marine_enrichment_medium" for consistent matching |
| 2026-05-16T09:03:31.705386+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:59.897694+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:38.145684+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:57:43.287931+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:56.784530+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:38:04.635378+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |