Overview
- External ontology
- TOGO:M412 — Modified SOB Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M412 Original source: JCM - JCM_M414 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=414
Composition
flowchart LR
CultureMech_009797["modified_sob_medium"]
ing0_Sol__1["Sol. 1"]
CultureMech_009797 --> ing0_Sol__1
ing1_Sol__2["Sol. 2"]
CultureMech_009797 --> ing1_Sol__2
ing2_Sol__3["Sol. 3"]
CultureMech_009797 --> ing2_Sol__3
ing3_HCl["HCl"]
CultureMech_009797 --> ing3_HCl
CHEBI_17883["CHEBI:17883"]
ing3_HCl -.-> CHEBI_17883
ing4_Distilled_water["Distilled water"]
CultureMech_009797 --> ing4_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Distilled_water -.-> CHEBI_15377
ing5_KH2PO4["KH2PO4"]
CultureMech_009797 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_NH4Cl["NH4Cl"]
CultureMech_009797 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_K2HPO4["K2HPO4"]
CultureMech_009797 --> ing7_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing7_K2HPO4 -.-> CHEBI_131527
ing8_Na2S2O3_5H2O["Na2S2O3・5H2O"]
CultureMech_009797 --> ing8_Na2S2O3_5H2O
CHEBI_32150["CHEBI:32150"]
ing8_Na2S2O3_5H2O -.-> CHEBI_32150
ing9_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009797 --> ing9_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_2H2O -.-> CHEBI_86158
ing10_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009797 --> ing10_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing10_MgCl2_6H2O -.-> CHEBI_86345
ing11_NaHCO3["NaHCO3"]
CultureMech_009797 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
ing12_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_009797 --> ing12_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_7H2O -.-> CHEBI_75836
ing13_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_009797 --> ing13_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing13_CoCl2_6H2O -.-> CHEBI_35696
ing14_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_009797 --> ing14_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_7H2O -.-> CHEBI_32312
ing15_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_009797 --> ing15_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing15_CuSO4_5H2O -.-> CHEBI_31440
ing16__NH4_Mo7O24_4H2O["(NH4)Mo7O24・4H2O"]
CultureMech_009797 --> ing16__NH4_Mo7O24_4H2O
ing17_EDTA_2Na["EDTA・2Na"]
CultureMech_009797 --> ing17_EDTA_2Na
CHEBI_64734["CHEBI:64734"]
ing17_EDTA_2Na -.-> CHEBI_64734
ing18_NaOH["NaOH"]
CultureMech_009797 --> ing18_NaOH
CHEBI_32145["CHEBI:32145"]
ing18_NaOH -.-> CHEBI_32145
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sol. 1 | 500 G_PER_L | ||
| 2 | Sol. 2 | 460 G_PER_L | ||
| 3 | Sol. 3 | 40 G_PER_L | ||
| 4 |
HCl
HCl 36.461 g/mol
|
variable VARIABLE |
CHEBI:17883
hydrogen chloride
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution |
| 5 |
Distilled water
H2O 18.015 g/mol
|
1001.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 500.0, 460.0, 40.0, 1.0] |
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Na2S2O3・5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1.55 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Inorganic compound, Simple component |
| 10 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
7.3999999999999995 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.1, 7.3] |
| 11 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.7 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.2, 2.5] |
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 13 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
5 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
0.5 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
2.2 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.2 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 | (NH4)Mo7O24・4H2O | 0.5 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 18 |
EDTA・2Na
C10H14N2O8.2Na 336.208 g/mol
|
50 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
| 19 |
NaOH
HO.Na 39.997 g/mol
|
11 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Simple component, Inorganic compound, Defined component |
Solutions
Trace metal solution (see below)
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.010526Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M412 |
| 2026-01-28T19:48:32.646207Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: HCl |
| 2026-03-09T08:04:54.204303+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:25.717699Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:20:08.959578Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009797 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.948784+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified SOB Medium" to "modified_sob_medium" for consistent matching |
| 2026-05-16T09:03:17.820802+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:41.172905+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:50.556034+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |