Overview
- External ontology
- TOGO:M41 — CYC Agar
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- TOGO M41 mirrors JCM Medium 49: modified Czapek-Dox liquid medium, yeast extract, casamino acids, and agar are brought to 1 L with distilled water, adjusted to pH 7.2, and autoclaved.
Composition
flowchart LR
CultureMech_009805["cyc_agar"]
ing0_Czapek_Dox_liquid_medium__modified__Oxoid_["Czapek-Dox liquid medium, modified (Oxoid)"]
CultureMech_009805 --> ing0_Czapek_Dox_liquid_medium__modified__Oxoid_
ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009805 --> ing1_Yeast_extract__BD_Difco_
ing2_Casamino_acids__vitamin_assay__BD_Difco_["Casamino acids, vitamin assay (BD-Difco)"]
CultureMech_009805 --> ing2_Casamino_acids__vitamin_assay__BD_Difco_
ing3_Agar["Agar"]
CultureMech_009805 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_Distilled_water["Distilled water"]
CultureMech_009805 --> ing4_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Distilled_water -.-> CHEBI_15377
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Czapek-Dox liquid medium, modified (Oxoid) | 33.4 G_PER_L | TOGO M41 and JCM Medium 49 list this component for CYC Agar; TOGO M212 and JCM Medium 219 use the same formulation adjusted to pH 8.0. | |
| 2 | Yeast extract (BD-Difco) | 2 G_PER_L | TOGO M41 and JCM Medium 49 list this component for CYC Agar; TOGO M212 and JCM Medium 219 use the same formulation adjusted to pH 8.0. | |
| 3 | Casamino acids, vitamin assay (BD-Difco) | 6 G_PER_L | TOGO M41 and JCM Medium 49 list this component for CYC Agar; TOGO M212 and JCM Medium 219 use the same formulation adjusted to pH 8.0. | |
| 4 | Agar | 16 G_PER_L |
CHEBI:2509
agar
|
TOGO M41 and JCM Medium 49 list this component for CYC Agar; TOGO M212 and JCM Medium 219 use the same formulation adjusted to pH 8.0. |
| 5 |
Distilled water
H2O 18.015 g/mol
|
1000 ML_PER_L |
CHEBI:15377
water
|
TOGO M41 and JCM Medium 49 list this component for CYC Agar; TOGO M212 and JCM Medium 219 use the same formulation adjusted to pH 8.0. |
Preparation
- Dissolve the CYC Agar components in distilled water and bring the final volume to 1 L.
- Adjust the complete medium to pH 7.2.
- Autoclave at 121 C for 15 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.005264Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M41 |
| 2026-01-28T19:48:09.863648Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:09.026236Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009805 |
| 2026-03-17T06:29:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.012788+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CYC Agar" to "cyc_agar" for consistent matching |
| 2026-06-05T07:40:41.227930+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-06T00:00:00-07:00 | repair_jcm_ph_score40.py | RESOLVED_JCM_PH_SCORE40_GRAPH | TOGO M41 mirrors JCM Medium 49: modified Czapek-Dox liquid medium, yeast extract, casamino acids, and agar are brought to 1 L with distilled water, adjusted to pH 7.2, and autoclaved. |
| 2026-09-06T00:00:00-07:00 | repair_jcm_ph_score40.py | LINKED_JCM_PH_SCORE40_CHILDREN | Added or refreshed reciprocal parent-child link for bacterial/TOGO_M212_CYC_Agar_pH_8.0.yaml. |