Overview
- External ontology
- TOGO:M420 — MMJHS Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M420 Original source: JCM - JCM_M421-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=421
Composition
flowchart LR
CultureMech_009806["mmjhs_medium"]
ing0_NaHCO3["NaHCO3"]
CultureMech_009806 --> ing0_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing0_NaHCO3 -.-> CHEBI_32139
ing1_NaNO3["NaNO3"]
CultureMech_009806 --> ing1_NaNO3
CHEBI_63005["CHEBI:63005"]
ing1_NaNO3 -.-> CHEBI_63005
ing2_Sulfur__powder_["Sulfur (powder)"]
CultureMech_009806 --> ing2_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing2_Sulfur__powder_ -.-> CHEBI_33403
ing3_Na2S2O3_5H2O["Na2S2O3・5H2O"]
CultureMech_009806 --> ing3_Na2S2O3_5H2O
CHEBI_32150["CHEBI:32150"]
ing3_Na2S2O3_5H2O -.-> CHEBI_32150
ing4_NaOH["NaOH"]
CultureMech_009806 --> ing4_NaOH
CHEBI_32145["CHEBI:32145"]
ing4_NaOH -.-> CHEBI_32145
ing5_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009806 --> ing5_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing5_Carbon_dioxide_gas -.-> CHEBI_16526
ing6_Hydrogen_gas["Hydrogen gas"]
CultureMech_009806 --> ing6_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing6_Hydrogen_gas -.-> CHEBI_18276
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaNO3
NO3.Na 84.994 g/mol
|
1 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 3 | Sulfur (powder) | 3 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 4 |
Na2S2O3・5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Inorganic compound, Simple component |
| 5 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 6 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 7 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: Gas |
Solutions
MJ(-N) synthetic seawater (see Medium [M260])
Dose: 1 G_PER_L
Cross-reference to Medium M260
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:55.028090Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M420 |
| 2026-01-28T19:48:23.194821Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:38.554922+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:09.031705Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009806 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.018480+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMJHS Medium" to "mmjhs_medium" for consistent matching |
| 2026-05-16T09:03:17.880785+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:41.233901+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |