Overview
- External ontology
- TOGO:M422 — Acidic Glycerol-Asparagine Agar (pH 5.0)
- Applications
-
- Microbial cultivation
- pH
- 5.0
- Notes
- TOGO M422/JCM Medium 422 prepares Glycerol-Asparagine Agar (ISP-5) from TOGO M52/JCM Medium 60 and adjusts the complete medium to pH 5.0.
Composition
flowchart LR
CultureMech_009808["acidic_glycerol_asparagine_agar_ph_5_0"]
ing0_L_Asparagine["L-Asparagine"]
CultureMech_009808 --> ing0_L_Asparagine
CHEBI_17196["CHEBI:17196"]
ing0_L_Asparagine -.-> CHEBI_17196
ing1_Glycerol["Glycerol"]
CultureMech_009808 --> ing1_Glycerol
CHEBI_17754["CHEBI:17754"]
ing1_Glycerol -.-> CHEBI_17754
ing2_K2HPO4["K2HPO4"]
CultureMech_009808 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Agar["Agar"]
CultureMech_009808 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_Distilled_water["Distilled water"]
CultureMech_009808 --> ing4_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Distilled_water -.-> CHEBI_15377
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
L-Asparagine
C4H8N2O3 132.119 g/mol
|
1 G_PER_L |
CHEBI:17196
L-asparagine
|
TOGO M52 and JCM Medium 60 list this component for Glycerol-Asparagine Agar (ISP-5); TOGO M422 and JCM Medium 422 use the same formulation adjusted to pH 5.0. |
| 2 |
Glycerol
C3H8O3 92.094 g/mol
|
10 G_PER_L |
CHEBI:17754
glycerol
|
TOGO M52 and JCM Medium 60 list this component for Glycerol-Asparagine Agar (ISP-5); TOGO M422 and JCM Medium 422 use the same formulation adjusted to pH 5.0. |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
TOGO M52 and JCM Medium 60 list this component for Glycerol-Asparagine Agar (ISP-5); TOGO M422 and JCM Medium 422 use the same formulation adjusted to pH 5.0. |
| 4 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
TOGO M52 and JCM Medium 60 list this component for Glycerol-Asparagine Agar (ISP-5); TOGO M422 and JCM Medium 422 use the same formulation adjusted to pH 5.0. |
| 5 |
Distilled water
H2O 18.015 g/mol
|
1000 ML_PER_L |
CHEBI:15377
water
|
TOGO M52 and JCM Medium 60 list this component for Glycerol-Asparagine Agar (ISP-5); TOGO M422 and JCM Medium 422 use the same formulation adjusted to pH 5.0. |
Preparation
- Dissolve the Glycerol-Asparagine Agar components in distilled water, add 1 mL Trace salts solution, and bring the final volume to 1 L.
- Adjust the complete medium to pH 5.0.
- Autoclave at 121 C for 15 min.
Solutions
Trace salts solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| FeSO4 x 7 H2O | 1 G_PER_L | JCM Medium 60 prepares Trace salts solution from 0.1 g each FeSO4 x 7 H2O, MnCl2 x 4 H2O, and ZnSO4 x 7 H2O in 100 mL distilled water, then adds 1 mL stock per final liter. |
| MnCl2 x 4 H2O | 1 G_PER_L | JCM Medium 60 prepares Trace salts solution from 0.1 g each FeSO4 x 7 H2O, MnCl2 x 4 H2O, and ZnSO4 x 7 H2O in 100 mL distilled water, then adds 1 mL stock per final liter. |
| ZnSO4 x 7 H2O | 1 G_PER_L | JCM Medium 60 prepares Trace salts solution from 0.1 g each FeSO4 x 7 H2O, MnCl2 x 4 H2O, and ZnSO4 x 7 H2O in 100 mL distilled water, then adds 1 mL stock per final liter. |
JCM Medium 60 prepares Trace salts solution from 0.1 g each FeSO4 x 7 H2O, MnCl2 x 4 H2O, and ZnSO4 x 7 H2O in 100 mL distilled water, then adds 1 mL stock per final liter.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.030454Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M422 |
| 2026-03-09T08:05:04.537133+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:09.042050Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009808 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T19:46:28.028520+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Acidic Glycerol-Asparagine Agar (pH 5.0)" to "acidic_glycerol_asparagine_agar_ph_5_0" for consistent matching |
| 2026-05-16T09:03:17.892962+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-09-06T00:00:00-07:00 | repair_jcm_ph_score40.py | RESOLVED_JCM_PH_SCORE40_GRAPH | TOGO M422/JCM Medium 422 prepares Glycerol-Asparagine Agar (ISP-5) from TOGO M52/JCM Medium 60 and adjusts the complete medium to pH 5.0. |