Overview
- External ontology
- TOGO:M423 — PYG Medium (B)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M423 Original source: JCM - JCM_M423 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=423
Composition
flowchart LR
CultureMech_009809["pyg_medium_b"]
ing0_Distilled_water["Distilled water"]
CultureMech_009809 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Glucose["Glucose"]
CultureMech_009809 --> ing1_Glucose
CHEBI_17234["CHEBI:17234"]
ing1_Glucose -.-> CHEBI_17234
ing2_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009809 --> ing2_Yeast_extract__BD_Difco_
ing3_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_009809 --> ing3_Tryptone__BD_Difco_
ing4_Polypepton__Nihon_Pharm__Co__["Polypepton (Nihon Pharm. Co.)"]
CultureMech_009809 --> ing4_Polypepton__Nihon_Pharm__Co__
ing5_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009809 --> ing5_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_7H2O -.-> CHEBI_31795
ing6_NaCl["NaCl"]
CultureMech_009809 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_KH2PO4["KH2PO4"]
CultureMech_009809 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8_K2HPO4["K2HPO4"]
CultureMech_009809 --> ing8_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4 -.-> CHEBI_131527
ing9_NaHCO3["NaHCO3"]
CultureMech_009809 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_CaCl2["CaCl2"]
CultureMech_009809 --> ing10_CaCl2
CHEBI_3312["CHEBI:3312"]
ing10_CaCl2 -.-> CHEBI_3312
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
961.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 960.0, 1.0] |
| 2 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 3 | Yeast extract (BD-Difco) | 10 G_PER_L | Properties: Undefined component | |
| 4 | Tryptone (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 5 | Polypepton (Nihon Pharm. Co.) | 5 G_PER_L | Properties: Undefined component | |
| 6 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
2 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound |
| 9 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 11 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.2 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
Solutions
Salts solution (see below)
Dose: 40 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.031069Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M423 |
| 2026-01-28T19:48:28.242428Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:46.792052+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:17.413701Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:09.048105Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009809 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.034130+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYG Medium (B)" to "pyg_medium_b" for consistent matching |
| 2026-05-16T09:03:17.899146+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:41.249303+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:30.426832+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.578026+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |