Overview
- External ontology
- TOGO:M442 — Modified Growth Medium With Pyruvate
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M442 Original source: JCM - JCM_M442 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=442
Composition
flowchart LR
CultureMech_009828["modified_growth_medium_with_pyruvate"]
ing0_Distilled_water["Distilled water"]
CultureMech_009828 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009828 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009828 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_009828 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009828 --> ing4_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_2H2O -.-> CHEBI_86158
ing5_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009828 --> ing5_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_6H2O -.-> CHEBI_86345
ing6_KCl["KCl"]
CultureMech_009828 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_009828 --> ing7_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing7_Sodium_pyruvate -.-> CHEBI_50144
ing8_Peptone__Oxoid_["Peptone (Oxoid)"]
CultureMech_009828 --> ing8_Peptone__Oxoid_
ing9_Tris_base["Tris base"]
CultureMech_009828 --> ing9_Tris_base
CHEBI_9754["CHEBI:9754"]
ing9_Tris_base -.-> CHEBI_9754
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
26.8 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
184 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.38 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
23 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
KCl
Cl.K 74.551 g/mol
|
5.4 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 8 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
1 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 9 | Peptone (Oxoid) | 5 G_PER_L | Properties: Undefined component | |
| 10 |
Tris base
C4H11NO3 121.136 g/mol
|
variable VARIABLE |
CHEBI:9754
tris
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.070014Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M442 |
| 2026-01-28T19:48:40.591237Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Tris base |
| 2026-03-13T06:20:09.216362Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009828 |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.192615+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Growth Medium With Pyruvate" to "modified_growth_medium_with_pyruvate" for consistent matching |
| 2026-05-16T09:03:18.062597+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:41.427272+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:30.566171+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |