Overview
- External ontology
- TOGO:M447 — Mjtso Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M447 Original source: JCM - JCM_M446 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=446
Composition
flowchart LR
CultureMech_009833["mjtso_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009833 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009833 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009833 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_009833 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_009833 --> ing4_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_2H2O -.-> CHEBI_86158
ing5_NH4Cl["NH4Cl"]
CultureMech_009833 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_K2HPO4["K2HPO4"]
CultureMech_009833 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_009833 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_KCl["KCl"]
CultureMech_009833 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_NaHCO3["NaHCO3"]
CultureMech_009833 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Sulfur__powder_["Sulfur (powder)"]
CultureMech_009833 --> ing10_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing10_Sulfur__powder_ -.-> CHEBI_33403
ing11_Na2S2O3_5H2O["Na2S2O3·5H2O"]
CultureMech_009833 --> ing11_Na2S2O3_5H2O
CHEBI_32150["CHEBI:32150"]
ing11_Na2S2O3_5H2O -.-> CHEBI_32150
ing12_NaOH["NaOH"]
CultureMech_009833 --> ing12_NaOH
CHEBI_32145["CHEBI:32145"]
ing12_NaOH -.-> CHEBI_32145
ing13_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009833 --> ing13_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing13_Carbon_dioxide_gas -.-> CHEBI_16526
ing14_Oxygen_gas["Oxygen gas"]
CultureMech_009833 --> ing14_Oxygen_gas
ing15_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_009833 --> ing15_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_2H2O -.-> CHEBI_75213
ing16_H3BO3["H3BO3"]
CultureMech_009833 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_009833 --> ing17_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing17_FeSO4_7H2O -.-> CHEBI_75836
ing18_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_009833 --> ing18_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing18_MnCl2_4H2O -.-> CHEBI_86368
ing19_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_009833 --> ing19_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing19_CoCl2_6H2O -.-> CHEBI_35696
ing20_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_009833 --> ing20_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing20_CuCl2_2H2O -.-> CHEBI_86318
ing21_ZnCl2["ZnCl2"]
CultureMech_009833 --> ing21_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing21_ZnCl2 -.-> CHEBI_49976
ing22_Na2WO4_2H2O["Na2WO4·2H2O"]
CultureMech_009833 --> ing22_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing22_Na2WO4_2H2O -.-> CHEBI_63939
ing23_NiSO4_6H2O["NiSO4·6H2O"]
CultureMech_009833 --> ing23_NiSO4_6H2O
CHEBI_53001["CHEBI:53001"]
ing23_NiSO4_6H2O -.-> CHEBI_53001
ing24_AlCl3_6H2O["AlCl3·6H2O"]
CultureMech_009833 --> ing24_AlCl3_6H2O
CHEBI_30115["CHEBI:30115"]
ing24_AlCl3_6H2O -.-> CHEBI_30115
ing25_H2SeO3["H2SeO3"]
CultureMech_009833 --> ing25_H2SeO3
CHEBI_26642["CHEBI:26642"]
ing25_H2SeO3 -.-> CHEBI_26642
ing26_EDTA_2Na["EDTA·2Na"]
CultureMech_009833 --> ing26_EDTA_2Na
CHEBI_64734["CHEBI:64734"]
ing26_EDTA_2Na -.-> CHEBI_64734
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.42 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 0.1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
3 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.07 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.14 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.34 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
KCl
Cl.K 74.551 g/mol
|
0.05 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 11 | Sulfur (powder) | 3 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Na2S2O3·5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Inorganic compound, Simple component |
| 13 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 14 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 15 | Oxygen gas | variable VARIABLE | Properties: Gas | |
| 16 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
0.15 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.02 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
Na2WO4·2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.03 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
NiSO4·6H2O
Ni.O4S 154.756 g/mol
|
0.02 G_PER_L |
CHEBI:53001
nickel sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
AlCl3·6H2O
AlCl3.6H2O 241.431 g/mol
|
0.04 G_PER_L |
CHEBI:30115
aluminium trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
H2SeO3
H2O3Se 128.973 g/mol
|
0.01 G_PER_L |
CHEBI:26642
selenous acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
EDTA·2Na
C10H14N2O8.2Na 336.208 g/mol
|
0.5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Simple component, Organic compound |
Solutions
Trace mineral solution (see below)
Dose: 10 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:55.077293Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M447 |
| 2026-01-28T19:48:20.345913Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Oxygen gas |
| 2026-03-09T08:04:33.715543+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:03.207740Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:09.252173Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009833 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.228179+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Mjtso Medium" to "mjtso_medium" for consistent matching |
| 2026-05-16T09:03:18.091298+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:41.466761+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:30.607173+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.656487+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:49.735609+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |