anoxic_medium_for_strain_acbe2_1 (CultureMech:009838)

Overview

External ontology

TOGO:M451 — Anoxic Medium For Strain AcBE2-1

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M451 Original source: JCM - JCM_M451 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=451

Composition

flowchart LR
    CultureMech_009838["anoxic_medium_for_strain_acbe2_1"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009838 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_009838 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_009838 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_009838 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_009838 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_009838 --> ing5_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_6H2O -.-> CHEBI_86345
    ing6_KCl["KCl"]
    CultureMech_009838 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_NaNO3["NaNO3"]
    CultureMech_009838 --> ing7_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing7_NaNO3 -.-> CHEBI_63005
    ing8_Na2SO4["Na2SO4"]
    CultureMech_009838 --> ing8_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing8_Na2SO4 -.-> CHEBI_32149
    ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009838 --> ing9_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing9_Carbon_dioxide_gas -.-> CHEBI_16526
    ing10_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009838 --> ing10_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing10_Nitrogen_gas -.-> CHEBI_17997

Ingredients (11)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
2	NaCl Cl.Na 58.443 g/mol	1 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3	CaCl2・2H2O Ca.2Cl.2H2O 147.014 g/mol	0.15 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
4	KH2PO4 H2O4P.K 136.084 g/mol	0.2 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
5	NH4Cl Cl.H4N 53.492 g/mol	0.25 G_PER_L	CHEBI:31206 ammonium chloride	Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
6	MgCl2・6H2O 2Cl.6H2O.Mg 203.301 g/mol	0.4 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
7	KCl Cl.K 74.551 g/mol	0.5 G_PER_L	CHEBI:32588 potassium chloride	Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
8	NaNO3 NO3.Na 84.994 g/mol	0.425 G_PER_L	CHEBI:63005 sodium nitrate	Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
9	Na2SO4 2Na.O4S 142.043 g/mol	0.07 G_PER_L	CHEBI:32149 sodium sulfate	Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
10	Carbon dioxide gas CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Properties: ガス
11	Nitrogen gas N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Properties: ガス

Solutions

84 g/L NaHCO3 solution (autoclaved under CO2 atmosphere)

Dose: 30 G_PER_L

Role: Buffer; Properties: Inorganic compound, Solution, Defined component

Trace element solution SL--10 (see Medium [M433])

Dose: 1 G_PER_L

Cross-reference to Medium M433

Selenite--tungstate solution (see Medium [M431])

Dose: 1 G_PER_L

Cross-reference to Medium M431

7 Vitamins solution (see Medium [M562])

Dose: 0.5 G_PER_L

Cross-reference to Medium M562

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:55.087203Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M451
2026-01-28T19:48:28.743712Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:47.641639+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 4 solution(s) from ingredients
2026-03-13T06:20:09.294028Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009838
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:26.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:28.267615+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Anoxic Medium For Strain AcBE2-1" to "anoxic_medium_for_strain_acbe2_1" for consistent matching
2026-05-16T09:03:18.133418+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:41.512084+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:50.674424+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.