1_5_nutrient_agar_no_2

CultureMech:009840 bacterial COMPLEX SOLID_AGAR
Original name: 1/5 Nutrient Agar NO. 2

Overview

External ontology
TOGO:M453 — 1/5 Nutrient Agar NO. 2
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M453 Original source: JCM - JCM_M453 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=453

Composition

flowchart LR
    CultureMech_009840["1_5_nutrient_agar_no_2"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009840 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_009840 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_Agar["Agar"]
    CultureMech_009840 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
    CultureMech_009840 --> ing3_Bacto_peptone__BD_Difco_
    ing4_Beef_extract__BD_Difco_["Beef extract (BD-Difco)"]
    CultureMech_009840 --> ing4_Beef_extract__BD_Difco_

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
3 Agar 15 G_PER_L CHEBI:2509
agar
 Role: Solidifying component
4 Bacto peptone (BD-Difco) 2 G_PER_L Properties: Undefined component
5 Beef extract (BD-Difco) 2 G_PER_L Properties: Undefined component

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.090120Z togo-import Imported from TOGO Medium Source: JCM, ID: M453
2026-01-28T19:48:17.331745Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:09.305116Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009840
2026-03-17T06:30:02.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:28.279047+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "1/5 Nutrient Agar NO. 2" to "1_5_nutrient_agar_no_2" for consistent matching
2026-06-05T07:40:41.518715+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.