Overview
- External ontology
- TOGO:M455 — Alkali-Reinforced Clostridial Agar
- Applications
-
- Microbial cultivation
- pH
- 10.0
- Notes
- JCM Medium 455 Alkali-Reinforced Clostridial Agar lists 51 g Reinforced clostridial agar (Sigma) in 900 ml distilled water per liter. After autoclaving at 121 C for 15 min, 100 ml of sterile 10% Na2CO3 solution is added aseptically and the final pH is checked to be about 10.0.
Composition
flowchart LR
CultureMech_009842["alkali_reinforced_clostridial_agar"]
ing0_Reinforced_clostridial_agar__Sigma_["Reinforced clostridial agar (Sigma)"]
CultureMech_009842 --> ing0_Reinforced_clostridial_agar__Sigma_
ing1_Distilled_water["Distilled water"]
CultureMech_009842 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Reinforced clostridial agar (Sigma) | 51.0 G_PER_L | TOGO M455 / JCM Medium 455 lists 51.0 g/L Reinforced clostridial agar (Sigma); this dehydrated commercial mixture is retained as an opaque complex component. | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
900.0 ML_PER_L |
CHEBI:15377
water
|
TOGO M455 / JCM Medium 455 lists 900.0 ml/L Distilled water. |
Preparation
- Autoclave the reinforced clostridial agar base at 121 C for 15 min.
- Aseptically add 100 ml/L sterile 10% Na2CO3 solution after autoclaving.
- Check final pH to be about 10.0.
Solutions
10% Na2CO3 solution
Dose: 100.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2CO3 | 10.0 PERCENT_W_V | JCM Medium 455 specifies a 10% Na2CO3 solution. |
TOGO M455 / JCM Medium 455 adds 100.0 ml/L sterile 10% Na2CO3 solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.093180Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M455 |
| 2026-01-28T19:48:28.615509Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:47.388742+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:09.319836Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009842 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:26.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.292576+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Alkali-Reinforced Clostridial Agar" to "alkali_reinforced_clostridial_agar" for consistent matching |
| 2026-05-16T09:03:18.153281+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:41.533560+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m455_score15.py | RESOLVED_TOGO_M455_SCORE15 | JCM Medium 455 Alkali-Reinforced Clostridial Agar lists 51 g Reinforced clostridial agar (Sigma) in 900 ml distilled water per liter. After autoclaving at 121 C for 15 min, 100 ml of sterile 10% Na2CO3 solution is added aseptically and the final pH is checked to be about 10.0. |