Overview
- External ontology
- TOGO:M460 — Fluid Thioglycollate Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M460 Original source: JCM - JCM_M460 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=460
Composition
flowchart LR
CultureMech_009848["fluid_thioglycollate_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009848 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_009848 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Resazurin["Resazurin"]
CultureMech_009848 --> ing2_Resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Resazurin -.-> CHEBI_8806
ing3_L__Cystine["L--Cystine"]
CultureMech_009848 --> ing3_L__Cystine
CHEBI_16283["CHEBI:16283"]
ing3_L__Cystine -.-> CHEBI_16283
ing4_Glucose["Glucose"]
CultureMech_009848 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Bacto_agar__BD_Difco___for_solid_medium_["Bacto agar (BD-Difco) (for solid medium)"]
CultureMech_009848 --> ing5_Bacto_agar__BD_Difco___for_solid_medium_
ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009848 --> ing6_Yeast_extract__BD_Difco_
ing7_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_009848 --> ing7_Trypticase_peptone__BD_BBL_
ing8_Sodium_thioglycollate["Sodium thioglycollate"]
CultureMech_009848 --> ing8_Sodium_thioglycollate
CHEBI_86481["CHEBI:86481"]
ing8_Sodium_thioglycollate -.-> CHEBI_86481
ing9_Na2CO3["Na2CO3"]
CultureMech_009848 --> ing9_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing9_Na2CO3 -.-> CHEBI_29377
ing10_N2["N2"]
CultureMech_009848 --> ing10_N2
CHEBI_17997["CHEBI:17997"]
ing10_N2 -.-> CHEBI_17997
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
2.5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component |
| 4 |
L--Cystine
C6H12N2O4S2 240.306 g/mol
|
0.5 G_PER_L |
CHEBI:16283
L-cystine
|
Role: Nitrogen source; Properties: Defined component, Simple component, Organic compound |
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
2 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 6 | Bacto agar (BD-Difco) (for solid medium) | 15 G_PER_L | Role: Solidifying component | |
| 7 | Yeast extract (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 8 | Trypticase peptone (BD-BBL) | 15 G_PER_L | Properties: Undefined component | |
| 9 |
Sodium thioglycollate
C2H3O2S.Na 114.101 g/mol
|
0.5 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
Properties: Defined component, Simple component, Organic compound |
| 10 |
Na2CO3
CO3.2Na 105.988 g/mol
|
variable VARIABLE |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Solution |
| 11 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.100194Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M460 |
| 2026-01-28T19:48:36.553744Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Na2CO3; Added default concentration for ingredient: N2 |
| 2026-03-13T06:20:09.357452Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009848 |
| 2026-03-17T06:30:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.327210+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Fluid Thioglycollate Medium" to "fluid_thioglycollate_medium" for consistent matching |
| 2026-05-16T09:03:18.181051+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:41.565034+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:50.688855+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-20T19:01:25.246559+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |