Overview
- External ontology
- TOGO:M461 — Chopped Meat Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M461 Original source: JCM - JCM_M461 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=461
Composition
flowchart LR
CultureMech_009849["chopped_meat_medium"]
ing0_1_N_NaOH["1 N NaOH"]
CultureMech_009849 --> ing0_1_N_NaOH
ing1_Distilled_water["Distilled water"]
CultureMech_009849 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_Ground_beef__fat_free_["Ground beef (fat free)"]
CultureMech_009849 --> ing2_Ground_beef__fat_free_
ing3_K2HPO4["K2HPO4"]
CultureMech_009849 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Resazurin["Resazurin"]
CultureMech_009849 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009849 --> ing5_Yeast_extract__BD_Difco_
ing6_Casitone__BD_Difco_["Casitone (BD-Difco)"]
CultureMech_009849 --> ing6_Casitone__BD_Difco_
ing7_L__cysteine_HCl_H2O["L--cysteine・HCl・H2O"]
CultureMech_009849 --> ing7_L__cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing7_L__cysteine_HCl_H2O -.-> CHEBI_91248
ing8_N2["N2"]
CultureMech_009849 --> ing8_N2
CHEBI_17997["CHEBI:17997"]
ing8_N2 -.-> CHEBI_17997
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 1 N NaOH | 25 G_PER_L | Properties: Defined component, Inorganic compound, Solution | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 3 | Ground beef (fat free) | 500 G_PER_L | Properties: Undefined component | |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component |
| 6 | Yeast extract (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 7 | Casitone (BD-Difco) | 30 G_PER_L | Properties: Undefined component | |
| 8 |
L--cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Organic compound, Simple component, Defined component |
| 9 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.101705Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M461 |
| 2026-01-28T19:48:27.546918Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-13T06:20:09.364512Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009849 |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.333498+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Chopped Meat Medium" to "chopped_meat_medium" for consistent matching |
| 2026-05-16T09:03:18.188058+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:41.571984+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:50.691823+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |