Overview
- External ontology
- TOGO:M482 — Reinforced Clostridial Medium With 3% Sea Salt
- Applications
-
- Microbial cultivation
- Notes
- TOGO M482 / JCM Medium 481 records TOGO M482 / JCM Medium 481 reinforced clostridial medium with 3% sea salt with 38 g/L Reinforced clostridial medium (BD-Difco), 30.0 g/L Sea salts (Sigma), 15 g/L Agar, 1 L distilled water, and the JCM default autoclaving at 121 C for 15 min. The commercial BD-Difco base and Sigma sea-salt mixture are retained as opaque unmapped components because JCM does not disclose their subcomposition.
Composition
flowchart LR
CultureMech_009869["reinforced_clostridial_medium_with_3_sea_salt"]
ing0_Reinforced_clostridial_medium__BD_Difco_["Reinforced clostridial medium (BD-Difco)"]
CultureMech_009869 --> ing0_Reinforced_clostridial_medium__BD_Difco_
ing1_Sea_salts__Sigma_["Sea salts (Sigma)"]
CultureMech_009869 --> ing1_Sea_salts__Sigma_
ing2_Agar["Agar"]
CultureMech_009869 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_Distilled_water["Distilled water"]
CultureMech_009869 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Reinforced clostridial medium (BD-Difco) | 38.0 G_PER_L | TOGO M482 / JCM Medium 481 lists 38 g/L commercial BD-Difco Reinforced Clostridial Medium but does not disclose its component recipe. | |
| 2 | Sea salts (Sigma) | 30.0 G_PER_L | TOGO M482 / JCM Medium 481 lists 30.0 g/L commercial Sigma sea salts; this heterogeneous salt mixture is retained as an opaque source-qualified component. | |
| 3 | Agar | 15.0 G_PER_L |
CHEBI:2509
agar
|
TOGO M482 / JCM Medium 481 lists 15.0 g/L Agar. |
| 4 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
TOGO M482 / JCM Medium 481 lists 1.0 L Distilled water. |
Preparation
- Autoclave at 121 C for 15 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.138631Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M482 |
| 2026-01-28T19:48:39.032703Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:09.528687Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009869 |
| 2026-03-17T06:30:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.495417+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Reinforced Clostridial Medium With 3% Sea Salt" to "reinforced_clostridial_medium_with_3_sea_salt" for consistent matching |
| 2026-06-05T07:40:41.763386+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-12T00:00:00-07:00 | repair_jcm_j481_j482_sea_salt_score15.py | RESOLVED_JCM_481_482_SEA_SALT_SCORE15 | Corrected the JCM 481/482 reinforced-clostridial sea-salt formula, grounded water and agar, added JCM default autoclaving, and marked the BD-Difco base and Sigma sea salts as intentionally opaque commercial ingredients. |