Overview
- External ontology
- TOGO:M491 — Mg50 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M491 Original source: JCM - JCM_M490 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=490
Composition
flowchart LR
CultureMech_009879["mg50_medium"]
ing0_Sol__1["Sol. 1"]
CultureMech_009879 --> ing0_Sol__1
ing1_distilled_water["distilled water"]
CultureMech_009879 --> ing1_distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_distilled_water -.-> CHEBI_15377
ing2_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009879 --> ing2_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_7H2O -.-> CHEBI_31795
ing3_Yeast_extract["Yeast extract"]
CultureMech_009879 --> ing3_Yeast_extract
ing4_NaCl["NaCl"]
CultureMech_009879 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009879 --> ing5_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_2H2O -.-> CHEBI_86158
ing6_KH2PO4["KH2PO4"]
CultureMech_009879 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7_NH4Cl["NH4Cl"]
CultureMech_009879 --> ing7_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing7_NH4Cl -.-> CHEBI_31206
ing8_K2HPO4["K2HPO4"]
CultureMech_009879 --> ing8_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4 -.-> CHEBI_131527
ing9_Resazurin["Resazurin"]
CultureMech_009879 --> ing9_Resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Resazurin -.-> CHEBI_8806
ing10_Casitone__BD_Difco_["Casitone (BD-Difco)"]
CultureMech_009879 --> ing10_Casitone__BD_Difco_
ing11_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009879 --> ing11_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing11_Carbon_dioxide_gas -.-> CHEBI_16526
ing12_Nitrogen_gas["Nitrogen gas"]
CultureMech_009879 --> ing12_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing12_Nitrogen_gas -.-> CHEBI_17997
ing13_Dithiothreitol["Dithiothreitol"]
CultureMech_009879 --> ing13_Dithiothreitol
CHEBI_18320["CHEBI:18320"]
ing13_Dithiothreitol -.-> CHEBI_18320
ing14_D__glucose["D--glucose"]
CultureMech_009879 --> ing14_D__glucose
ing15_N2["N2"]
CultureMech_009879 --> ing15_N2
ing15_N2 -.-> CHEBI_17997
ing16_NaHCO3["NaHCO3"]
CultureMech_009879 --> ing16_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing16_NaHCO3 -.-> CHEBI_32139
ing17_H3BO3["H3BO3"]
CultureMech_009879 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_009879 --> ing18_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing18_MnCl2_4H2O -.-> CHEBI_86368
ing19_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_009879 --> ing19_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing19_CoCl2_6H2O -.-> CHEBI_35696
ing20_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_009879 --> ing20_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing20_NiCl2_6H2O -.-> CHEBI_34887
ing21_CuCl2_2H2O["CuCl2・2H2O"]
CultureMech_009879 --> ing21_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing21_CuCl2_2H2O -.-> CHEBI_86318
ing22_ZnCl2["ZnCl2"]
CultureMech_009879 --> ing22_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing22_ZnCl2 -.-> CHEBI_49976
ing23_FeCl3_6H2O["FeCl3・6H2O"]
CultureMech_009879 --> ing23_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing23_FeCl3_6H2O -.-> CHEBI_86254
ing24_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009879 --> ing24_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing24_Nitrilotriacetic_acid -.-> CHEBI_44557
ing25_KOH["KOH"]
CultureMech_009879 --> ing25_KOH
CHEBI_32035["CHEBI:32035"]
ing25_KOH -.-> CHEBI_32035
Ingredients (26)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sol. 1 | 912 G_PER_L | ||
| 2 |
distilled water
H2O 18.015 g/mol
|
1010.98 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 5 duplicates: 900.0, 10.0, 19.98, 80.0, 1.0] |
| 3 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 | Yeast extract | 0.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
3.25 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 2.25, 1.0] |
| 6 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.35 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.25, 0.1] |
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.227 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.348 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 11 | Casitone (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 12 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 13 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 14 |
Dithiothreitol
C4H10O2S2 154.256 g/mol
|
0.3 G_PER_L |
CHEBI:18320
1,4-dithiothreitol
|
Properties: Defined component, Inorganic compound, Simple component |
| 15 | D--glucose | 3.6 G_PER_L | Role: Carbon source; Properties: Defined component, Organic compound, Simple component | |
| 16 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 17 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
0.024 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
0.12 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
CuCl2・2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.025 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
FeCl3・6H2O
Cl3Fe.6H2O 270.294 g/mol
|
1.35 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
12.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 26 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
Solutions
dithiothreitol solution
Dose: 10 G_PER_L
glucose solution
Dose: 19.98 G_PER_L
NaHCO3 solution
Dose: 80 G_PER_L
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Trace element solution (see Medium No. [480])
Dose: 1 G_PER_L
Se/W solution (see Medium [M312])
Dose: 1 G_PER_L
Cross-reference to Medium M312
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.153740Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M491 |
| 2026-01-28T19:48:08.719249Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: KOH |
| 2026-03-09T08:04:14.047511+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T03:08:41.070537Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 6 duplicate ingredient(s) |
| 2026-03-13T06:20:09.599116Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009879 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.562790+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Mg50 Medium" to "mg50_medium" for consistent matching |
| 2026-05-16T09:03:18.436759+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:41.858116+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:30.899300+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.764788+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |