Overview
- External ontology
- TOGO:M494 — MMJ-HO Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M494 Original source: JCM - JCM_M493 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=493
Composition
flowchart LR
CultureMech_009882["mmj_ho_medium"]
ing0_NaHCO3["NaHCO3"]
CultureMech_009882 --> ing0_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing0_NaHCO3 -.-> CHEBI_32139
ing1_NaNO3["NaNO3"]
CultureMech_009882 --> ing1_NaNO3
CHEBI_63005["CHEBI:63005"]
ing1_NaNO3 -.-> CHEBI_63005
ing2_Sulfur__powder_["Sulfur (powder)"]
CultureMech_009882 --> ing2_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing2_Sulfur__powder_ -.-> CHEBI_33403
ing3_NaOH["NaOH"]
CultureMech_009882 --> ing3_NaOH
CHEBI_32145["CHEBI:32145"]
ing3_NaOH -.-> CHEBI_32145
ing4_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009882 --> ing4_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing4_Carbon_dioxide_gas -.-> CHEBI_16526
ing5_N2["N2"]
CultureMech_009882 --> ing5_N2
CHEBI_17997["CHEBI:17997"]
ing5_N2 -.-> CHEBI_17997
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaNO3
NO3.Na 84.994 g/mol
|
1 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 3 | Sulfur (powder) | 3 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 4 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 5 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 6 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Solutions
Modified MJ synthetic seawater (see Medium [M493])
Dose: 1 G_PER_L
Cross-reference to Medium M493
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:55.160975Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M494 |
| 2026-01-28T19:48:34.567395Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:57.416116+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:09.625544Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009882 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.588637+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMJ-HO Medium" to "mmj_ho_medium" for consistent matching |
| 2026-05-16T09:03:18.467062+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:41.891575+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |