modified_eichler_and_pfennigs_medium

CultureMech:009886 bacterial COMPLEX LIQUID
Original name: Modified Eichler And Pfennig's Medium

Overview

External ontology
TOGO:M498 — Modified Eichler And Pfennig's Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M498 Original source: JCM - JCM_M497 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=497

Composition

flowchart LR
    CultureMech_009886["modified_eichler_and_pfennigs_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009886 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_009886 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_009886 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_009886 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_009886 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_NH4Cl["NH4Cl"]
    CultureMech_009886 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_Ammonium_acetate["Ammonium acetate"]
    CultureMech_009886 --> ing6_Ammonium_acetate
    CHEBI_62947["CHEBI:62947"]
    ing6_Ammonium_acetate -.-> CHEBI_62947
    ing7_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_009886 --> ing7_Yeast_extract__BD_Difco_
    ing8_NaHCO3["NaHCO3"]
    CultureMech_009886 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_Vitamin_B12__2_mg_100_ml_["Vitamin B12 (2 mg/100 ml)"]
    CultureMech_009886 --> ing9_Vitamin_B12__2_mg_100_ml_
    ing10_Na2S_9H2O__final_concentration_["Na2S・9H2O (final concentration)"]
    CultureMech_009886 --> ing10_Na2S_9H2O__final_concentration_

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 NaCl
Cl.Na 58.443 g/mol
20 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
4 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.25 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
5 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Simple component, Defined component, Inorganic compound
6 NH4Cl
Cl.H4N 53.492 g/mol
0.68 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
7 Ammonium acetate
C2H3O2.H4N 77.083 g/mol
0.5 G_PER_L CHEBI:62947
ammonium acetate
 Role: Nitrogen source; Properties: Defined component, Simple component, Organic compound
8 Yeast extract (BD-Difco) 0.5 G_PER_L Properties: Undefined component
9 NaHCO3
CHO3.Na 84.006 g/mol
1.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
10 Vitamin B12 (2 mg/100 ml) 1 G_PER_L Role: Vitamin; Properties: Organic compound, Solution, Defined component
11 Na2S・9H2O (final concentration) variable VARIABLE Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution

Solutions

Trace element solution SL--12 (see Medium [M497])

Dose: 1 G_PER_L

Cross-reference to Medium M497

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.166942Z togo-import Imported from TOGO Medium Source: JCM, ID: M498
2026-01-28T19:48:36.841130Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Na2S・9H2O (final concentration)
2026-03-09T08:05:01.493375+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:09.656169Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009886
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:28.617941+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Eichler And Pfennig's Medium" to "modified_eichler_and_pfennigs_medium" for consistent matching
2026-05-16T09:03:18.501098+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:41.925723+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:30.957195+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:50.782632+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.