Overview
- External ontology
- TOGO:M4 — Lactobacillus Medium I
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M4 Original source: JCM - JCM_M11 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=11
Composition
flowchart LR
CultureMech_009889["lactobacillus_medium_i"]
ing0_Distilled_water["Distilled water"]
CultureMech_009889 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Tween_80["Tween 80"]
CultureMech_009889 --> ing1_Tween_80
CHEBI_53426["CHEBI:53426"]
ing1_Tween_80 -.-> CHEBI_53426
ing2_Tomato_juice__filtered__pH_7_0["Tomato juice, filtered, pH 7.0"]
CultureMech_009889 --> ing2_Tomato_juice__filtered__pH_7_0
ing3_Lactose["Lactose"]
CultureMech_009889 --> ing3_Lactose
CHEBI_36218["CHEBI:36218"]
ing3_Lactose -.-> CHEBI_36218
ing4_Glucose["Glucose"]
CultureMech_009889 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Agar["Agar"]
CultureMech_009889 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009889 --> ing6_Yeast_extract__BD_Difco_
ing7_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_009889 --> ing7_Tryptone__BD_Difco_
ing8_Tryptose__BD_Difco_["Tryptose (BD-Difco)"]
CultureMech_009889 --> ing8_Tryptose__BD_Difco_
ing9_Liver_extract_concentrate__NBCo_["Liver extract concentrate (NBCo)"]
CultureMech_009889 --> ing9_Liver_extract_concentrate__NBCo_
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
50 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Simple component, Organic compound, Defined component |
| 3 | Tomato juice, filtered, pH 7.0 | 200 G_PER_L | Role: Growth factor; Properties: Undefined component | |
| 4 |
Lactose
C12H22O11 342.297 g/mol
|
2 G_PER_L |
CHEBI:36218
beta-lactose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
3 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 7 | Yeast extract (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 8 | Tryptone (BD-Difco) | 20 G_PER_L | Properties: Undefined component | |
| 9 | Tryptose (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 10 | Liver extract concentrate (NBCo) | 1 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.986480Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M4 |
| 2026-01-28T19:48:34.257506Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:09.672933Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009889 |
| 2026-03-17T06:30:37.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.634324+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Lactobacillus Medium I" to "lactobacillus_medium_i" for consistent matching |
| 2026-06-05T07:40:41.938634+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |