inorganic_salts_starch_agar_isp_4

CultureMech:009900 bacterial COMPLEX SOLID_AGAR
Original name: Inorganic Salts-Starch Agar (ISP-4)

Overview

External ontology
TOGO:M50 — Inorganic Salts-Starch Agar (ISP-4)
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 7.4, 'notes': 'JCM Medium 58 says the unadjusted pH will be 7.0-7.4.'}
Notes
TOGO M50 mirrors JCM Medium 58: Inorganic Salts-Starch Agar (ISP-4) combines soluble starch, inorganic salts, 1 mL/L Trace salts solution, agar, and distilled water; its unadjusted pH is 7.0-7.4.

Composition

flowchart LR
    CultureMech_009900["inorganic_salts_starch_agar_isp_4"]
    ing0_Soluble_starch["Soluble starch"]
    CultureMech_009900 --> ing0_Soluble_starch
    CHEBI_28017["CHEBI:28017"]
    ing0_Soluble_starch -.-> CHEBI_28017
    ing1_K2HPO4["K2HPO4"]
    CultureMech_009900 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_009900 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NaCl["NaCl"]
    CultureMech_009900 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_009900 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_CaCO3["CaCO3"]
    CultureMech_009900 --> ing5_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing5_CaCO3 -.-> CHEBI_3311
    ing6_Agar["Agar"]
    CultureMech_009900 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Distilled_water["Distilled water"]
    CultureMech_009900 --> ing7_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing7_Distilled_water -.-> CHEBI_15377

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Soluble starch 10.0 G_PER_L CHEBI:28017
starch
 JCM Medium 58 prints this component for ISP-4.
2 K2HPO4
HO4P.2K 174.174 g/mol
1.0 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 JCM Medium 58 prints this component for ISP-4.
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1.0 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 JCM Medium 58 prints this component for ISP-4.
4 NaCl
Cl.Na 58.443 g/mol
1.0 G_PER_L CHEBI:26710
sodium chloride
 JCM Medium 58 prints this component for ISP-4.
5 (NH4)2SO4
2H4N.O4S 132.141 g/mol
2.0 G_PER_L CHEBI:62946
ammonium sulfate
 JCM Medium 58 prints this component for ISP-4.
6 CaCO3
CO3.Ca 100.086 g/mol
2.0 G_PER_L CHEBI:3311
calcium carbonate
 JCM Medium 58 prints this component for ISP-4.
7 Agar 20.0 G_PER_L CHEBI:2509
agar
 JCM Medium 58 prints this component for ISP-4.
8 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 JCM Medium 58 prints this component for ISP-4.

Preparation

  1. Mix the JCM Medium 58 ISP-4 ingredients with 1 mL/L Trace salts solution.
  2. Autoclave at 121 C for 15 min.

Solutions

Trace salts solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 1 G_PER_L JCM Medium 58 prepares Trace salts solution from 0.1 g each FeSO4 x 7 H2O, MnCl2 x 4 H2O, and ZnSO4 x 7 H2O in 100 mL distilled water, then adds 1 mL stock per final liter.
MnCl2 x 4 H2O 1 G_PER_L JCM Medium 58 prepares Trace salts solution from 0.1 g each FeSO4 x 7 H2O, MnCl2 x 4 H2O, and ZnSO4 x 7 H2O in 100 mL distilled water, then adds 1 mL stock per final liter.
ZnSO4 x 7 H2O 1 G_PER_L JCM Medium 58 prepares Trace salts solution from 0.1 g each FeSO4 x 7 H2O, MnCl2 x 4 H2O, and ZnSO4 x 7 H2O in 100 mL distilled water, then adds 1 mL stock per final liter.
JCM Medium 58 prepares Trace salts solution from 0.1 g each FeSO4 x 7 H2O, MnCl2 x 4 H2O, and ZnSO4 x 7 H2O in 100 mL distilled water, then adds 1 mL stock per final liter.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.170882Z togo-import Imported from TOGO Medium Source: JCM, ID: M50
2026-01-28T19:48:07.099552Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:11.448712+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T03:08:38.172989Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:20:09.791820Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009900
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:28.745008+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Inorganic Salts-Starch Agar (ISP-4)" to "inorganic_salts_starch_agar_isp_4" for consistent matching
2026-05-16T09:03:18.606777+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:42.037221+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:31.046001+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:50.824337+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-06T00:00:00-07:00 repair_jcm_isp_supplements_score40.py RESOLVED_JCM_ISP_SUPPLEMENTS_SCORE40_GRAPH TOGO M50 mirrors JCM Medium 58: Inorganic Salts-Starch Agar (ISP-4) combines soluble starch, inorganic salts, 1 mL/L Trace salts solution, agar, and distilled water; its unadjusted pH is 7.0-7.4.
2026-09-06T00:00:00-07:00 repair_jcm_isp_supplements_score40.py LINKED_JCM_ISP_SUPPLEMENTS_SCORE40_CHILDREN JCM Medium 217 supplements JCM Medium 58 ISP-4 with 0.5 g/L Yeast extract.
2026-09-08T00:00:00-07:00 repair_jcm_salinity_score20.py LINKED_JCM_SALINITY_SCORE20_CHILDREN Added or refreshed reciprocal salinity-variant link for bacterial/TOGO_M1238_Inorganic_Salts-Starch_Agar_ISP-4_With_5_NaCl.yaml.
2026-09-08T00:00:00-07:00 repair_jcm_salinity_score20.py LINKED_JCM_SALINITY_SCORE20_CHILDREN Added or refreshed reciprocal salinity-variant link for bacterial/TOGO_M346_Inorganic_Salts-Starch_Agar_ISP-4_With_10_NaCl.yaml.
2026-09-08T00:00:00-07:00 repair_jcm_salinity_score20.py LINKED_JCM_SALINITY_SCORE20_CHILDREN Added or refreshed reciprocal salinity-variant link for bacterial/TOGO_M900_Inorganic_Salts-Starch_Agar_ISP-4_With_15_NaCl.yaml.
2026-09-13T00:00:00-07:00 repair_official_simple_backlinks.py ADDED_OFFICIAL_SIMPLE_BACKLINKS Mirrored curated official-simple parent_media links on the parent record.