glucose_sulfide_medium (CultureMech:009906)

Overview

External ontology

TOGO:M515 — Glucose Sulfide Medium

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M515 Original source: JCM - JCM_M514 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=514

Composition

flowchart LR
    CultureMech_009906["glucose_sulfide_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009906 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_009906 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_009906 --> ing2_Yeast_extract
    ing3_K2HPO4["K2HPO4"]
    CultureMech_009906 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_009906 --> ing4_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing4_Na2S_9H2O -.-> CHEBI_76209
    ing5_KCl["KCl"]
    CultureMech_009906 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6__NH4_2SO4["(NH4)2SO4"]
    CultureMech_009906 --> ing6__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing6__NH4_2SO4 -.-> CHEBI_62946
    ing7_CaCO3["CaCO3"]
    CultureMech_009906 --> ing7_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing7_CaCO3 -.-> CHEBI_3311
    ing8_Ca_NO3_2["Ca(NO3)2"]
    CultureMech_009906 --> ing8_Ca_NO3_2
    CHEBI_64205["CHEBI:64205"]
    ing8_Ca_NO3_2 -.-> CHEBI_64205
    ing9_Glucose["Glucose"]
    CultureMech_009906 --> ing9_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing9_Glucose -.-> CHEBI_17234
    ing10_Agar["Agar"]
    CultureMech_009906 --> ing10_Agar
    CHEBI_2509["CHEBI:2509"]
    ing10_Agar -.-> CHEBI_2509
    ing11_Biotin["Biotin"]
    CultureMech_009906 --> ing11_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing11_Biotin -.-> CHEBI_15956
    ing12_p__Aminobenzoic_acid["p--Aminobenzoic acid"]
    CultureMech_009906 --> ing12_p__Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing12_p__Aminobenzoic_acid -.-> CHEBI_30753
    ing13_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_009906 --> ing13_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing13_Calcium_pantothenate -.-> CHEBI_31345
    ing14_Pyridoxine_HCl["Pyridoxine・HCl"]
    CultureMech_009906 --> ing14_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing14_Pyridoxine_HCl -.-> CHEBI_30961
    ing15_Folic_acid["Folic acid"]
    CultureMech_009906 --> ing15_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing15_Folic_acid -.-> CHEBI_27470
    ing16_Vitamin_B12["Vitamin B12"]
    CultureMech_009906 --> ing16_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing16_Vitamin_B12 -.-> CHEBI_176843
    ing17_Riboflavin["Riboflavin"]
    CultureMech_009906 --> ing17_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing17_Riboflavin -.-> CHEBI_17015
    ing18_Nicotinic_acid["Nicotinic acid"]
    CultureMech_009906 --> ing18_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing18_Nicotinic_acid -.-> CHEBI_15940
    ing19_Thiamine_HCl_2H2O["Thiamine・HCl・2H2O"]
    CultureMech_009906 --> ing19_Thiamine_HCl_2H2O
    ing20_myo__Inositol["myo--Inositol"]
    CultureMech_009906 --> ing20_myo__Inositol
    CHEBI_17268["CHEBI:17268"]
    ing20_myo__Inositol -.-> CHEBI_17268

Ingredients (21)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	991.0 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 990.0, 1.0]
2	MgSO4・7H2O 7H2O.Mg.O4S 246.473 g/mol	0.05 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3	Yeast extract	1 G_PER_L		Role: Nutrient source; Properties: Undefined component
4	K2HPO4 HO4P.2K 174.174 g/mol	0.05 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5	Na2S・9H2O 9H2O.2Na.S 240.182 g/mol	0.2 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component
6	KCl Cl.K 74.551 g/mol	0.05 G_PER_L	CHEBI:32588 potassium chloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
7	(NH4)2SO4 2H4N.O4S 132.141 g/mol	0.5 G_PER_L	CHEBI:62946 ammonium sulfate	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
8	CaCO3 CO3.Ca 100.086 g/mol	0.1 G_PER_L	CHEBI:3311 calcium carbonate	Role: Protective agent, Mineral source; Properties: Inorganic compound, Defined component, Simple component
9	Ca(NO3)2 Ca.2NO3 164.086 g/mol	0.1 G_PER_L	CHEBI:64205 calcium nitrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10	Glucose C6H12O6 180.156 g/mol	0.15 G_PER_L	CHEBI:17234 glucose	Role: Carbon source; Properties: Organic compound, Defined component, Simple component
11	Agar	15 G_PER_L	CHEBI:2509 agar	Role: Solidifying component
12	Biotin C10H16N2O3S 244.316 g/mol	2 G_PER_L	CHEBI:15956 biotin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
13	p--Aminobenzoic acid C7H7NO2 137.138 g/mol	1 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
14	Calcium pantothenate 2C9H16NO5.Ca 476.536 g/mol	5 G_PER_L	CHEBI:31345 Calcium pantothenate	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
15	Pyridoxine・HCl C8H11NO3.HCl 205.641 g/mol	10 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
16	Folic acid C19H19N7O6 441.404 g/mol	2 G_PER_L	CHEBI:27470 folic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
17	Vitamin B12	0.01 G_PER_L	CHEBI:176843 vitamin B12	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
18	Riboflavin C17H20N4O6 376.369 g/mol	5 G_PER_L	CHEBI:17015 riboflavin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
19	Nicotinic acid C6H5NO2 123.111 g/mol	5 G_PER_L	CHEBI:15940 nicotinic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
20	Thiamine・HCl・2H2O	5 G_PER_L		Role: Vitamin; Properties: Organic compound, Defined component, Simple component
21	myo--Inositol C6H12O6 180.156 g/mol	1 G_PER_L	CHEBI:17268 myo-inositol	Role: Growth factor; Properties: Organic compound, Defined component, Simple component

Solutions

Vitamin solution (see below)

Dose: 10 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:55.211258Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M515
2026-01-28T19:48:40.131954Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:05:07.621903+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 1 solution(s) from ingredients
2026-03-13T03:09:39.340650Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 1 duplicate ingredient(s)
2026-03-13T06:20:09.858743Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009906
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name
2026-03-17T06:30:51.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:28.807045+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Glucose Sulfide Medium" to "glucose_sulfide_medium" for consistent matching
2026-05-16T09:03:18.682498+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-06-05T07:40:42.110319+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:34.463000+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (audit G21/G22)	Re-grounded 3 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T06:57:31.117133+00:00	mgso4-heptahydrate-term-fix-v1.0	Corrected MgSO4·7H2O primary term grounding to heptahydrate	Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:50.850671+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.