modified_desulfoglaeba_alkanexedens_medium

CultureMech:009914 bacterial COMPLEX LIQUID
Original name: Modified Desulfoglaeba Alkanexedens Medium

Overview

External ontology
TOGO:M522 — Modified Desulfoglaeba Alkanexedens Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M522 Original source: JCM - JCM_M521 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=521

Composition

flowchart LR
    CultureMech_009914["modified_desulfoglaeba_alkanexedens_medium"]
    ing0_Mineral_medium["Mineral medium"]
    CultureMech_009914 --> ing0_Mineral_medium
    ing1_Sol__1["Sol. 1"]
    CultureMech_009914 --> ing1_Sol__1
    ing2_Distilled_water["Distilled water"]
    CultureMech_009914 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377
    ing3_NH4Cl["NH4Cl"]
    CultureMech_009914 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_Resazurin["Resazurin"]
    CultureMech_009914 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5_Na2SO4["Na2SO4"]
    CultureMech_009914 --> ing5_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing5_Na2SO4 -.-> CHEBI_32149
    ing6_Sea_salts__Sigma_["Sea salts (Sigma)"]
    CultureMech_009914 --> ing6_Sea_salts__Sigma_
    ing7_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009914 --> ing7_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing7_Carbon_dioxide_gas -.-> CHEBI_16526
    ing8_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009914 --> ing8_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing8_Nitrogen_gas -.-> CHEBI_17997
    ing9_Decane["Decane"]
    CultureMech_009914 --> ing9_Decane
    CHEBI_41808["CHEBI:41808"]
    ing9_Decane -.-> CHEBI_41808
    ing10_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_009914 --> ing10_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_9H2O -.-> CHEBI_76209
    ing11_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_009914 --> ing11_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing11_L__Cysteine_HCl_H2O -.-> CHEBI_91248
    ing12_N2["N2"]
    CultureMech_009914 --> ing12_N2
    ing12_N2 -.-> CHEBI_17997

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Mineral medium 9 G_PER_L
2 Sol. 1 1.14 G_PER_L
3 Distilled water
H2O 18.015 g/mol
901.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Merged 2 duplicates: 900.0, 1.0]
4 NH4Cl
Cl.H4N 53.492 g/mol
0.25 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
5 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component
6 Na2SO4
2Na.O4S 142.043 g/mol
3.5 G_PER_L CHEBI:32149
sodium sulfate
 Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
7 Sea salts (Sigma) 25 G_PER_L Role: Mineral source; Properties: Complex component, Defined component
8 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
9 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
10 Decane
C10H22 142.286 g/mol
0.01 G_PER_L CHEBI:41808
decane
 Role: Carbon source; Properties: Defined component, Simple component, Organic compound
11 Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
12.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Simple component, Inorganic compound, Defined component
12 L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
12.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Organic compound, Simple component
13 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス

Solutions

8.5% NaHCO3*

Dose: 0.4 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

1% yeast extract solution

Dose: 0.05 G_PER_L

Role: Nutrient source; Properties: Undefined component

2% Sodium pyruvate solution*

Dose: 0.5 G_PER_L

Role: Carbon source; Properties: Solution, Defined component, Organic compound

Trace vitamins (see Medium [M190])

Dose: 0.1 G_PER_L

Cross-reference to Medium M190

Reducing agent solution (see below)

Dose: 0.08 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.227746Z togo-import Imported from TOGO Medium Source: JCM, ID: M522
2026-01-28T19:48:09.189775Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2
2026-03-09T08:04:14.815535+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 5 solution(s) from ingredients
2026-03-13T03:08:41.957478Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:20:09.927685Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009914
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:28.872862+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Desulfoglaeba Alkanexedens Medium" to "modified_desulfoglaeba_alkanexedens_medium" for consistent matching
2026-05-16T09:03:18.759151+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:42.189451+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:50.876443+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.