Overview
- External ontology
- TOGO:M525 — Methane Oxidizing Bacterium Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M525 Original source: JCM - JCM_M524 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=524
Composition
flowchart LR
CultureMech_009917["methane_oxidizing_bacterium_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009917 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009917 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_009917 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009917 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009917 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_009917 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009917 --> ing6_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_6H2O -.-> CHEBI_86345
ing7_KCl["KCl"]
CultureMech_009917 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_009917 --> ing8_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing8_NiCl2_6H2O -.-> CHEBI_34887
ing9_Na2SeO3_5H2O["Na2SeO3・5H2O"]
CultureMech_009917 --> ing9_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing9_Na2SeO3_5H2O -.-> CHEBI_131361
ing10_NaNO3["NaNO3"]
CultureMech_009917 --> ing10_NaNO3
CHEBI_63005["CHEBI:63005"]
ing10_NaNO3 -.-> CHEBI_63005
ing11_Na2SiO3_9H2O["Na2SiO3・9H2O"]
CultureMech_009917 --> ing11_Na2SiO3_9H2O
CHEBI_132108["CHEBI:132108"]
ing11_Na2SiO3_9H2O -.-> CHEBI_132108
ing12_Fe2_SO4_3_xH2O["Fe2(SO4)3・xH2O"]
CultureMech_009917 --> ing12_Fe2_SO4_3_xH2O
ing13_O2["O2"]
CultureMech_009917 --> ing13_O2
ing14_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009917 --> ing14_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing14_Carbon_dioxide_gas -.-> CHEBI_16526
ing15_Nitrogen_gas["Nitrogen gas"]
CultureMech_009917 --> ing15_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing15_Nitrogen_gas -.-> CHEBI_17997
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.8 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.14 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 7 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 9 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
0.5 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 10 |
Na2SeO3・5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.5 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 11 |
NaNO3
NO3.Na 84.994 g/mol
|
0.25 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 12 |
Na2SiO3・9H2O
9H2O.2Na.O3Si 284.198 g/mol
|
0.2 G_PER_L |
CHEBI:132108
sodium silicate nonahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 13 | Fe2(SO4)3・xH2O | 20 G_PER_L | Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound | |
| 14 | O2 | variable VARIABLE | Properties: Gas | |
| 15 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 16 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
Trace mineral solution (see Medium [M524])
Dose: 3 G_PER_L
Cross-reference to Medium M524
5% NaHCO3 solution
Dose: 10 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
1 mM CuSO4 solution
Dose: 2 G_PER_L
Role: Mineral source
Trace vitamins (see Medium [M190])
Dose: 1 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.233539Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M525 |
| 2026-01-28T19:48:28.293345Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: O2; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:46.872415+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T06:20:09.954985Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009917 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.897953+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Methane Oxidizing Bacterium Medium" to "methane_oxidizing_bacterium_medium" for consistent matching |
| 2026-05-16T09:03:18.788156+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:42.216545+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:31.205776+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.887390+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |