glycerol_asparagine_agar_isp_5

CultureMech:009922 bacterial COMPLEX SOLID_AGAR
Original name: Glycerol-Asparagine Agar (ISP-5)

Overview

External ontology
TOGO:M52 — Glycerol-Asparagine Agar (ISP-5)
Applications
  • Microbial cultivation
pH
7.4
Notes
TOGO M52 mirrors JCM Medium 60: L-asparagine, glycerol, K2HPO4, 1 mL Trace salts solution, and agar are brought to 1 L with distilled water, adjusted to pH 7.4, and autoclaved. The Trace salts solution stock is modeled as a solution rather than as undiluted final-liter ingredients.

Composition

flowchart LR
    CultureMech_009922["glycerol_asparagine_agar_isp_5"]
    ing0_L_Asparagine["L-Asparagine"]
    CultureMech_009922 --> ing0_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing0_L_Asparagine -.-> CHEBI_17196
    ing1_Glycerol["Glycerol"]
    CultureMech_009922 --> ing1_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing1_Glycerol -.-> CHEBI_17754
    ing2_K2HPO4["K2HPO4"]
    CultureMech_009922 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Agar["Agar"]
    CultureMech_009922 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Distilled_water["Distilled water"]
    CultureMech_009922 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 L-Asparagine
C4H8N2O3 132.119 g/mol
1 G_PER_L CHEBI:17196
L-asparagine
 TOGO M52 and JCM Medium 60 list this component for Glycerol-Asparagine Agar (ISP-5); TOGO M422 and JCM Medium 422 use the same formulation adjusted to pH 5.0.
2 Glycerol
C3H8O3 92.094 g/mol
10 G_PER_L CHEBI:17754
glycerol
 TOGO M52 and JCM Medium 60 list this component for Glycerol-Asparagine Agar (ISP-5); TOGO M422 and JCM Medium 422 use the same formulation adjusted to pH 5.0.
3 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 TOGO M52 and JCM Medium 60 list this component for Glycerol-Asparagine Agar (ISP-5); TOGO M422 and JCM Medium 422 use the same formulation adjusted to pH 5.0.
4 Agar 15 G_PER_L CHEBI:2509
agar
 TOGO M52 and JCM Medium 60 list this component for Glycerol-Asparagine Agar (ISP-5); TOGO M422 and JCM Medium 422 use the same formulation adjusted to pH 5.0.
5 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 TOGO M52 and JCM Medium 60 list this component for Glycerol-Asparagine Agar (ISP-5); TOGO M422 and JCM Medium 422 use the same formulation adjusted to pH 5.0.

Preparation

  1. Dissolve the Glycerol-Asparagine Agar components in distilled water, add 1 mL Trace salts solution, and bring the final volume to 1 L.
  2. Adjust the complete medium to pH 7.4.
  3. Autoclave at 121 C for 15 min.

Solutions

Trace salts solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 1 G_PER_L JCM Medium 60 prepares Trace salts solution from 0.1 g each FeSO4 x 7 H2O, MnCl2 x 4 H2O, and ZnSO4 x 7 H2O in 100 mL distilled water, then adds 1 mL stock per final liter.
MnCl2 x 4 H2O 1 G_PER_L JCM Medium 60 prepares Trace salts solution from 0.1 g each FeSO4 x 7 H2O, MnCl2 x 4 H2O, and ZnSO4 x 7 H2O in 100 mL distilled water, then adds 1 mL stock per final liter.
ZnSO4 x 7 H2O 1 G_PER_L JCM Medium 60 prepares Trace salts solution from 0.1 g each FeSO4 x 7 H2O, MnCl2 x 4 H2O, and ZnSO4 x 7 H2O in 100 mL distilled water, then adds 1 mL stock per final liter.
JCM Medium 60 prepares Trace salts solution from 0.1 g each FeSO4 x 7 H2O, MnCl2 x 4 H2O, and ZnSO4 x 7 H2O in 100 mL distilled water, then adds 1 mL stock per final liter.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.219914Z togo-import Imported from TOGO Medium Source: JCM, ID: M52
2026-01-28T19:48:19.318500Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:31.986690+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T03:09:01.177320Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:20:09.984749Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009922
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:06.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:28.926708+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Glycerol-Asparagine Agar (ISP-5)" to "glycerol_asparagine_agar_isp_5" for consistent matching
2026-05-16T09:03:18.814088+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:42.243079+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:34.503663+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:50.899133+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-06T00:00:00-07:00 repair_jcm_ph_score40.py RESOLVED_JCM_PH_SCORE40_GRAPH TOGO M52 mirrors JCM Medium 60: L-asparagine, glycerol, K2HPO4, 1 mL Trace salts solution, and agar are brought to 1 L with distilled water, adjusted to pH 7.4, and autoclaved. The Trace salts solution stock is modeled as a solution rather than as undiluted final-liter ingredients.
2026-09-06T00:00:00-07:00 repair_jcm_ph_score40.py LINKED_JCM_PH_SCORE40_CHILDREN Added or refreshed reciprocal parent-child link for bacterial/TOGO_M422_Acidic_Glycerol-Asparagine_Agar_pH_5.0.yaml.
2026-09-08T00:00:00-07:00 repair_jcm_isp5_salinity_score20.py LINKED_JCM_ISP5_SALINITY_SCORE20_CHILDREN Added or refreshed reciprocal salinity-variant link for bacterial/TOGO_M408_Glycerol-Asparagine_Agar_ISP-5_With_10_NaCl.yaml.