brucella_broth

Overview

External ontology

TOGO:M540 — Brucella Broth

Applications

• Microbial cultivation

Notes

Source: https://togomedium.org/medium/M540 Original source: JCM - JCM_M538 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=538

Composition

flowchart LR
    CultureMech_009933["brucella_broth"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009933 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_009933 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_009933 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Glucose["Glucose"]
    CultureMech_009933 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_NaHSO3["NaHSO3"]
    CultureMech_009933 --> ing4_NaHSO3
    CHEBI_26709["CHEBI:26709"]
    ing4_NaHSO3 -.-> CHEBI_26709
    ing5_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_009933 --> ing5_Pancreatic_digest_of_casein
    ing6_Peptic_digest_of_animal_tissue["Peptic digest of animal tissue"]
    CultureMech_009933 --> ing6_Peptic_digest_of_animal_tissue

Ingredients (7)

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

• Preferred term

  Helicobacter pylori

  Term

  Id

  NCBITaxon:210

  Label

  Helicobacter pylori

  Strain

  OMU89-362

  Growth metrics

  • Max od600

    0.724

    Max od wavelength nm

    620

    Temperature celsius

    37

    Measurement conditions

    Bisulfite-less Brucella broth supplemented with 10% fetal bovine serum, pretreated with 3.5 mM H2O2 and catalase; source compared this condition with standard bisulfite-containing Brucella broth and H2O2-supplemented Brucella broth.

    Evidence

    • Reference

      PMID:10609610

      Supports

      SUPPORT

      Snippet

      bacteria grew to a similar extent in bisulfite-less Brucella broth (BLBB0)/FBS compared to the bacterial growth in BHIB/FBS

      Explanation

      The source abstract supports growth in the Brucella-broth-derived variant; manual full-text review provides the OD620 value and identifies Helicobacter pylori OMU89-362 as the primary strain in the experiment.

    Is max attainment

    True

  Evidence

  • Reference

    PMID:10609610

    Supports

    SUPPORT

    Snippet

    The enhancement was evident at 0.1 mM HPOx and reached a maximun at 3.5 mM.

    Explanation

    The source names Helicobacter pylori OMU89-362 and directly reports enhanced growth in Brucella broth supplemented with fetal bovine serum and hydrogen peroxide.

Recipe variants

• Name

  brucella_broth_fbs_h2o2_helicobacter_pylori

  Description

  Brucella broth condition used for Helicobacter pylori liquid growth, with fetal bovine serum supplementation and hydrogen-peroxide detoxification of the bisulfite-containing parent broth.

  Modifications

  • Supplement Brucella broth with 10% fetal bovine serum.
  • Add hydrogen peroxide at sublethal concentrations, with maximum growth stimulation reported at 3.5 mM H2O2.
  • Incubate microaerobically at 37 C with shaking; source measured OD620 and viable CFU after 24 h.

  Purpose

  Capture strain-specific Helicobacter pylori growth evidence under the shared Brucella broth parent while modeling serum and H2O2 as study-specific variant conditions.

  Evidence

  • Reference

    PMID:10609610

    Supports

    SUPPORT

    Snippet

    enhanced the growth of Helicobacter pylori in Brucella broth supplemented with 10% fetal bovine serum (BB/FBS)

    Explanation

    The source uses the same Brucella broth basal formulation, supplemented with fetal bovine serum and hydrogen peroxide, to measure Helicobacter pylori OMU89-362 growth.

Provenance