Overview
- External ontology
- TOGO:M542 — Modified Syntrophomonas Medium For OL4
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M542 Original source: JCM - JCM_M540 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=540
Composition
flowchart LR
CultureMech_009935["modified_syntrophomonas_medium_for_ol4"]
ing0_Na2S_9H2O["Na2S・9H2O"]
CultureMech_009935 --> ing0_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing0_Na2S_9H2O -.-> CHEBI_76209
ing1_L__cysteine_HCl_H2O["L--cysteine・HCl・H2O"]
CultureMech_009935 --> ing1_L__cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing1_L__cysteine_HCl_H2O -.-> CHEBI_91248
ing2_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009935 --> ing2_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing2_Carbon_dioxide_gas -.-> CHEBI_16526
ing3_N2["N2"]
CultureMech_009935 --> ing3_N2
CHEBI_17997["CHEBI:17997"]
ing3_N2 -.-> CHEBI_17997
ing4_Distilled_water["Distilled water"]
CultureMech_009935 --> ing4_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Distilled_water -.-> CHEBI_15377
ing5_Resazurin["Resazurin"]
CultureMech_009935 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_Na2SO4["Na2SO4"]
CultureMech_009935 --> ing6_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing6_Na2SO4 -.-> CHEBI_32149
ing7_Nitrogen_gas["Nitrogen gas"]
CultureMech_009935 --> ing7_Nitrogen_gas
ing7_Nitrogen_gas -.-> CHEBI_17997
ing8_Sodium_oleate["Sodium oleate"]
CultureMech_009935 --> ing8_Sodium_oleate
CHEBI_81860["CHEBI:81860"]
ing8_Sodium_oleate -.-> CHEBI_81860
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.3 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
L--cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.3 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Organic compound, Simple component |
| 3 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 4 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 5 |
Distilled water
H2O 18.015 g/mol
|
845.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 745.0, 100.0] |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Simple component, Defined component, Organic compound |
| 7 |
Na2SO4
2Na.O4S 142.043 g/mol
|
2.8 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 8 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 9 |
Sodium oleate
C18H33O2.Na 304.45 g/mol
|
0.15 G_PER_L |
CHEBI:81860
Sodium oleate
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
Solutions
NaHCO3 solution
Dose: 100 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Solution A
Dose: 802 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 100 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Mineral solution (see Medium [M520])
Dose: 50 G_PER_L
Cross-reference to Medium M520
FeCl2 solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Trace element solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Vitamin solution (see Medium [M520])
Dose: 5 G_PER_L
Cross-reference to Medium M520
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.263111Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M542 |
| 2026-01-28T19:48:22.593662Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:37.677716+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 7 solution(s) from ingredients |
| 2026-03-13T03:09:07.377997Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:10.088413Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009935 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:13.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:29.023563+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Syntrophomonas Medium For OL4" to "modified_syntrophomonas_medium_for_ol4" for consistent matching |
| 2026-05-16T09:03:18.910802+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:42.353675+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:50.932245+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |