desulfotomaculum_lam5_medium

CultureMech:009940 bacterial COMPLEX LIQUID
Original name: Desulfotomaculum Lam5 Medium

Overview

External ontology
TOGO:M547 — Desulfotomaculum Lam5 Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M547 Original source: JCM - JCM_M545 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=545

Composition

flowchart LR
    CultureMech_009940["desulfotomaculum_lam5_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009940 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_009940 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_009940 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KH2PO4["KH2PO4"]
    CultureMech_009940 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_009940 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_K2HPO4["K2HPO4"]
    CultureMech_009940 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_Resazurin["Resazurin"]
    CultureMech_009940 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_009940 --> ing7_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_6H2O -.-> CHEBI_86345
    ing8_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_009940 --> ing8_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing8_Na2S_9H2O -.-> CHEBI_76209
    ing9_KCl["KCl"]
    CultureMech_009940 --> ing9_KCl
    CHEBI_32588["CHEBI:32588"]
    ing9_KCl -.-> CHEBI_32588
    ing10_NaHCO3["NaHCO3"]
    CultureMech_009940 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_Na2SO4["Na2SO4"]
    CultureMech_009940 --> ing11_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing11_Na2SO4 -.-> CHEBI_32149
    ing12_CaCl2["CaCl2"]
    CultureMech_009940 --> ing12_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing12_CaCl2 -.-> CHEBI_3312
    ing13_Sodium_lactate["Sodium lactate"]
    CultureMech_009940 --> ing13_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing13_Sodium_lactate -.-> CHEBI_75228
    ing14_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_009940 --> ing14_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing14_L__Cysteine_HCl_H2O -.-> CHEBI_91248
    ing15_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009940 --> ing15_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing15_Carbon_dioxide_gas -.-> CHEBI_16526
    ing16_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009940 --> ing16_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing16_Nitrogen_gas -.-> CHEBI_17997

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
2 Yeast extract 0.1 G_PER_L Role: Nutrient source; Properties: Undefined component
3 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
6 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
7 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component
8 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.5 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
9 Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
0.4 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component
10 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11 NaHCO3
CHO3.Na 84.006 g/mol
2 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
12 Na2SO4
2Na.O4S 142.043 g/mol
3 G_PER_L CHEBI:32149
sodium sulfate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
13 CaCl2
Ca.2Cl 110.984 g/mol
0.1 G_PER_L CHEBI:3312
calcium dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
14 Sodium lactate
C3H5O3.Na 112.06 g/mol
1 G_PER_L CHEBI:75228
sodium lactate
 Role: Carbon source; Properties: Organic compound, Defined component, Simple component
15 L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Organic compound, Simple component
16 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
17 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス

Solutions

Trace element solution (see Medium [M439])

Dose: 10 G_PER_L

Cross-reference to Medium M439

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.273740Z togo-import Imported from TOGO Medium Source: JCM, ID: M547
2026-01-28T19:48:07.314670Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:11.800224+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:10.139616Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009940
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:29.071020+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Desulfotomaculum Lam5 Medium" to "desulfotomaculum_lam5_medium" for consistent matching
2026-05-16T09:03:18.957021+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:42.400057+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:50.950690+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.