Overview
- External ontology
- TOGO:M548 — Halophile Yeast Extract Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M548 Original source: JCM - JCM_M546 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=546
Composition
flowchart LR
CultureMech_009941["halophile_yeast_extract_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009941 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009941 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009941 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_009941 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009941 --> ing4_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_2H2O -.-> CHEBI_86158
ing5_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009941 --> ing5_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_6H2O -.-> CHEBI_86345
ing6_KCl["KCl"]
CultureMech_009941 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_NaHCO3["NaHCO3"]
CultureMech_009941 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_NaBr["NaBr"]
CultureMech_009941 --> ing8_NaBr
CHEBI_63004["CHEBI:63004"]
ing8_NaBr -.-> CHEBI_63004
ing9_NaOH["NaOH"]
CultureMech_009941 --> ing9_NaOH
CHEBI_32145["CHEBI:32145"]
ing9_NaOH -.-> CHEBI_32145
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
50.8 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 3 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
195 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.8 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
32.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
KCl
Cl.K 74.551 g/mol
|
5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.16 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 9 |
NaBr
Br.Na 102.894 g/mol
|
0.6 G_PER_L |
CHEBI:63004
sodium bromide
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 10 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.275989Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M548 |
| 2026-01-28T19:48:34.145812Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-13T06:20:10.147669Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009941 |
| 2026-03-17T06:30:37.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:29.079134+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Halophile Yeast Extract Medium" to "halophile_yeast_extract_medium" for consistent matching |
| 2026-05-16T09:03:36.842259+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:08.009283+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:48.461956+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:59.598662+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |