Overview
- External ontology
- TOGO:M54 — Glycerol-Calcium Malate Agar
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M54 Original source: JCM - JCM_M62 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=62
Composition
flowchart LR
CultureMech_009943["glycerol_calcium_malate_agar"]
ing0_Distilled_water["Distilled water"]
CultureMech_009943 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NH4Cl["NH4Cl"]
CultureMech_009943 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_K2HPO4["K2HPO4"]
CultureMech_009943 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Glycerol["Glycerol"]
CultureMech_009943 --> ing3_Glycerol
CHEBI_17754["CHEBI:17754"]
ing3_Glycerol -.-> CHEBI_17754
ing4_Calcium_malate["Calcium malate"]
CultureMech_009943 --> ing4_Calcium_malate
ing5_Agar["Agar"]
CultureMech_009943 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
Glycerol
C3H8O3 92.094 g/mol
|
10 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 5 | Calcium malate | 10 G_PER_L | Role: Mineral source; Properties: Simple component, Organic compound, Defined component | |
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.258133Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M54 |
| 2026-01-28T19:48:40.411883Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:10.160235Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009943 |
| 2026-03-17T06:30:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:29.091695+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Glycerol-Calcium Malate Agar" to "glycerol_calcium_malate_agar" for consistent matching |
| 2026-06-05T07:40:42.416029+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:34.588981+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:50.958780+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |