geosporobacter_subterreneus_medium

CultureMech:009944 bacterial COMPLEX LIQUID
Original name: Geosporobacter Subterreneus Medium

Overview

External ontology
TOGO:M550 — Geosporobacter Subterreneus Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M550 Original source: JCM - JCM_M548 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=548

Composition

flowchart LR
    CultureMech_009944["geosporobacter_subterreneus_medium"]
    ing0_Sol__1["Sol. 1"]
    CultureMech_009944 --> ing0_Sol__1
    ing1_Distilled_water["Distilled water"]
    CultureMech_009944 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2_NH4Cl["NH4Cl"]
    CultureMech_009944 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_Resazurin["Resazurin"]
    CultureMech_009944 --> ing3_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Resazurin -.-> CHEBI_8806
    ing4_Sea_salts__Sigma_["Sea salts (Sigma)"]
    CultureMech_009944 --> ing4_Sea_salts__Sigma_
    ing5_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009944 --> ing5_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing5_Carbon_dioxide_gas -.-> CHEBI_16526
    ing6_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009944 --> ing6_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing6_Nitrogen_gas -.-> CHEBI_17997
    ing7_1_M_Glucose["1 M Glucose"]
    CultureMech_009944 --> ing7_1_M_Glucose

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Sol. 1 0.4 G_PER_L
2 Distilled water
H2O 18.015 g/mol
920 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
3 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
4 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component
5 Sea salts (Sigma) 10 G_PER_L Role: Mineral source; Properties: Defined component, Complex component
6 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
7 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
8 1 M Glucose 0.1 G_PER_L Role: Carbon source; Properties: Defined component, Organic compound, Solution

Solutions

Solution A

Dose: 4.6 G_PER_L

See mediadive_5342_Solution_A.yaml for composition

5% NaHCO3 solution

Dose: 0.2 G_PER_L

Role: Buffer; Properties: Defined component, Solution, Inorganic compound

10% Yeast extract solution

Dose: 0.05 G_PER_L

Role: Nutrient source; Properties: Undefined component

2% Na2S・9H2O solution

Dose: 0.05 G_PER_L

Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.280552Z togo-import Imported from TOGO Medium Source: JCM, ID: M550
2026-01-28T19:48:15.719748Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:25.883836+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 4 solution(s) from ingredients
2026-03-13T03:08:54.400347Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:20:10.166967Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009944
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:29.097999+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Geosporobacter Subterreneus Medium" to "geosporobacter_subterreneus_medium" for consistent matching
2026-05-16T09:03:18.977568+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:42.423070+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:50.961133+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.