sulfate_reducing_bacterium_medium_with_lactate

CultureMech:009949 bacterial COMPLEX SOLID_AGAR
Original name: Sulfate Reducing Bacterium Medium With Lactate

Overview

External ontology
TOGO:M555 — Sulfate Reducing Bacterium Medium With Lactate
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M555 Original source: JCM - JCM_M552-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=552

Composition

flowchart LR
    CultureMech_009949["sulfate_reducing_bacterium_medium_with_lactate"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009949 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_009949 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_009949 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_009949 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_009949 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_NH4Cl["NH4Cl"]
    CultureMech_009949 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_Resazurin["Resazurin"]
    CultureMech_009949 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_Na2SO4["Na2SO4"]
    CultureMech_009949 --> ing7_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing7_Na2SO4 -.-> CHEBI_32149
    ing8_L__Sodium_lactate["L--Sodium lactate"]
    CultureMech_009949 --> ing8_L__Sodium_lactate
    ing9_agar__BD_Difco_["agar (BD-Difco)"]
    CultureMech_009949 --> ing9_agar__BD_Difco_
    ing10_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_009949 --> ing10_Yeast_extract__BD_Difco_
    ing11_L__Cysteine_H2O_HCl["L--Cysteine・H2O・HCl"]
    CultureMech_009949 --> ing11_L__Cysteine_H2O_HCl
    ing12_NaOH["NaOH"]
    CultureMech_009949 --> ing12_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing12_NaOH -.-> CHEBI_32145
    ing13_N2["N2"]
    CultureMech_009949 --> ing13_N2
    CHEBI_17997["CHEBI:17997"]
    ing13_N2 -.-> CHEBI_17997

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 NaCl
Cl.Na 58.443 g/mol
10 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
6 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component
7 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound
8 Na2SO4
2Na.O4S 142.043 g/mol
1 G_PER_L CHEBI:32149
sodium sulfate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
9 L--Sodium lactate 2.3 G_PER_L Role: Carbon source; Properties: Defined component, Simple component, Organic compound
10 agar (BD-Difco) 15 G_PER_L Role: Solidifying component
11 Yeast extract (BD-Difco) 0.5 G_PER_L Properties: Undefined component
12 L--Cysteine・H2O・HCl 0.5 G_PER_L Properties: Defined component, Simple component, Organic compound
13 NaOH
HO.Na 39.997 g/mol
variable VARIABLE CHEBI:32145
sodium hydroxide
 Properties: Defined component, Inorganic compound, Solution
14 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas

Solutions

Vitamin solution (see Medium [M554])

Dose: 1 G_PER_L

Cross-reference to Medium M554

FeCl2 solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Trace element solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.290843Z togo-import Imported from TOGO Medium Source: JCM, ID: M555
2026-01-28T19:48:08.634235Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: NaOH; Added default concentration for ingredient: N2
2026-03-09T08:04:13.940876+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T06:20:10.217192Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009949
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:29.147583+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Sulfate Reducing Bacterium Medium With Lactate" to "sulfate_reducing_bacterium_medium_with_lactate" for consistent matching
2026-05-16T09:03:19.033312+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:42.480809+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:31.405577+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:50.983999+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.