ilyobacter_medium

CultureMech:009966 bacterial COMPLEX LIQUID
Original name: Ilyobacter Medium

Overview

External ontology
TOGO:M570 — Ilyobacter Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M570 Original source: JCM - JCM_M566 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=566

Composition

flowchart LR
    CultureMech_009966["ilyobacter_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009966 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_009966 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_009966 --> ing2_Yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_009966 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_009966 --> ing4_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_2H2O -.-> CHEBI_86158
    ing5_NH4Cl["NH4Cl"]
    CultureMech_009966 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_KCl["KCl"]
    CultureMech_009966 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_Sodium_acetate["Sodium acetate"]
    CultureMech_009966 --> ing7_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing7_Sodium_acetate -.-> CHEBI_32954
    ing8_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_009966 --> ing8_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing8_Sodium_pyruvate -.-> CHEBI_50144
    ing9_KNO3["KNO3"]
    CultureMech_009966 --> ing9_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing9_KNO3 -.-> CHEBI_63043
    ing10_Sodium_succinate["Sodium succinate"]
    CultureMech_009966 --> ing10_Sodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing10_Sodium_succinate -.-> CHEBI_63675
    ing11_KH2PO4_2H2O["KH2PO4・2H2O"]
    CultureMech_009966 --> ing11_KH2PO4_2H2O
    ing12_Glucose["Glucose"]
    CultureMech_009966 --> ing12_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing12_Glucose -.-> CHEBI_17234
    ing13_L_Cysteine_HCl_H2O["L-Cysteine・HCl・H2O"]
    CultureMech_009966 --> ing13_L_Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing13_L_Cysteine_HCl_H2O -.-> CHEBI_91248
    ing14_DL__sodium_Malate["DL--sodium Malate"]
    CultureMech_009966 --> ing14_DL__sodium_Malate
    ing15_Peptone["Peptone"]
    CultureMech_009966 --> ing15_Peptone
    ing16_Na2S["Na2S"]
    CultureMech_009966 --> ing16_Na2S
    CHEBI_76208["CHEBI:76208"]
    ing16_Na2S -.-> CHEBI_76208
    ing17_N2["N2"]
    CultureMech_009966 --> ing17_N2
    CHEBI_17997["CHEBI:17997"]
    ing17_N2 -.-> CHEBI_17997

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
2 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 Yeast extract 0.5 G_PER_L Role: Nutrient source; Properties: Undefined component
4 NaCl
Cl.Na 58.443 g/mol
20 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
5 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.02 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
6 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
7 KCl
Cl.K 74.551 g/mol
0.77 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
8 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.1 G_PER_L CHEBI:32954
sodium acetate
 Role: Buffer; Properties: Defined component, Organic compound, Simple component
9 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
0.1 G_PER_L CHEBI:50144
sodium pyruvate
 Role: Carbon source; Properties: Defined component, Simple component, Organic compound
10 KNO3
K.NO3 101.102 g/mol
1 G_PER_L CHEBI:63043
potassium nitrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
11 Sodium succinate
C4H4O4.2Na 162.052 g/mol
0.1 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
 Role: Growth factor; Properties: Organic compound, Simple component, Defined component
12 KH2PO4・2H2O 0.5 G_PER_L Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
13 Glucose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Simple component, Organic compound, Defined component
14 L-Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.3 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Organic compound, Simple component
15 DL--sodium Malate 0.1 G_PER_L Properties: Defined component, Simple component, Organic compound
16 Peptone 0.5 G_PER_L
17 Na2S
2Na.S 78.047 g/mol
variable VARIABLE CHEBI:76208
sodium sulfide (anhydrous)
 Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution
18 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas

Solutions

Resazurin solution (1%)

Dose: 100 G_PER_L

Role: pH dependent redox indicator; Properties: Defined component, Solution

Wolfe's mineral solution (see Medium [M257])

Dose: 10 G_PER_L

Cross-reference to Medium M257

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.323814Z togo-import Imported from TOGO Medium Source: JCM, ID: M570
2026-01-28T19:48:13.506982Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Na2S; Added default concentration for ingredient: N2
2026-03-09T08:04:22.327769+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T06:20:10.360872Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009966
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:29.290804+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Ilyobacter Medium" to "ilyobacter_medium" for consistent matching
2026-05-16T09:03:19.190007+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:42.634988+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:31.539027+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:51.043115+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.