modified_halobacteria_medium

CultureMech:009972 archaea COMPLEX LIQUID
Original name: Modified Halobacteria Medium

Overview

External ontology
TOGO:M576 — Modified Halobacteria Medium
Applications
  • Microbial cultivation
pH
7.5
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M576 Original source: JCM - JCM_M572 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=572 Ingredient amounts use the exact formulation and preparation stage. Separate preparation excerpt: "Add components to distilled water and bring volume to 1.0 L.". Conditional solid option: "For preparation of solid medium, add 20.0 g/L agar.". Agar is included only in M372/M960 in this reviewed variant cohort; no step ordering is invented. The inherited base pH range does not replace the source-explicit post-autoclave pH 7.5 target.

Composition

flowchart LR
    CultureMech_009972["modified_halobacteria_medium"]
    ing0_distilled_water["distilled water"]
    CultureMech_009972 --> ing0_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_009972 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_009972 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KCl["KCl"]
    CultureMech_009972 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_MnCl2_4H2O["MnCl2・4H2O"]
    CultureMech_009972 --> ing4_MnCl2_4H2O
    CHEBI_86368["CHEBI:86368"]
    ing4_MnCl2_4H2O -.-> CHEBI_86368
    ing5_Sodium_glutamate["Sodium glutamate"]
    CultureMech_009972 --> ing5_Sodium_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing5_Sodium_glutamate -.-> CHEBI_64220
    ing6_Trisodium_citrate["Trisodium citrate"]
    CultureMech_009972 --> ing6_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing6_Trisodium_citrate -.-> CHEBI_53258
    ing7_FeCl2_4H2O["FeCl2・4H2O"]
    CultureMech_009972 --> ing7_FeCl2_4H2O
    CHEBI_86249["CHEBI:86249"]
    ing7_FeCl2_4H2O -.-> CHEBI_86249
    ing8_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_009972 --> ing8_Yeast_extract__BD_Difco_
    ing9_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
    CultureMech_009972 --> ing9_Casamino_acids__BD_Difco_

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 distilled water
H2O 18.015 g/mol
CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component Water makes the preparation up to 1.0 L. Its separate input volume and mass are unspecified; neither 1 g/L nor a fixed 1 L water dose is supported.
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component Use the recipe-specific final formulation, not the copied base amount. The source explicitly gives this final g/L target.
3 NaCl
Cl.Na 58.443 g/mol
200 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound 200.0 g in the source preparation brought to 1.0 L gives 200 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
4 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component 2.0 g in the source preparation brought to 1.0 L gives 2 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
5 MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
0.36 MG_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component 0.36 mg in the source preparation brought to 1.0 L gives 0.36 MG_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
6 Sodium glutamate
C5H8NO4.Na 169.112 g/mol
1 G_PER_L CHEBI:64220
monosodium glutamate
 Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component 1.0 g in the source preparation brought to 1.0 L gives 1 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
7 Trisodium citrate
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
 Role: Buffer; Properties: Defined component, Organic compound, Simple component 3.0 g in the source preparation brought to 1.0 L gives 3 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
8 FeCl2・4H2O
Cl2Fe.4H2O 198.811 g/mol
36 MG_PER_L CHEBI:86249
iron dichloride tetrahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component 36.0 mg in the source preparation brought to 1.0 L gives 36 MG_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
9 Yeast extract (BD-Difco) 5 G_PER_L Properties: Undefined component 5.0 g in the source preparation brought to 1.0 L gives 5 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
10 Casamino acids (BD-Difco) 5 G_PER_L Properties: Undefined component 5.0 g in the source preparation brought to 1.0 L gives 5 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".

Solutions

Na2CO3 solution

Dose: variable VARIABLE

Role: Buffer; Properties: Defined component, Inorganic compound, Solution Sterile sodium carbonate solution adjusts the medium to pH 7.5 after autoclaving. Dose and stock strength are unspecified; retain any source-explicit variable dose without inventing a numeric concentration.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.333847Z togo-import Imported from TOGO Medium Source: JCM, ID: M576
2026-01-28T19:48:11.810265Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Na2CO3 solution
2026-03-09T08:04:19.456126+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:10.402408Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009972
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:29.333229+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Halobacteria Medium" to "modified_halobacteria_medium" for consistent matching
2026-05-16T09:03:19.229099+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:42.672142+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:31.579046+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-10-09T14:23:51.976655+00:00 codex CURATED_HALOBACTERIA_VARIANT_CONCENTRATIONS_AND_PREPARATION_STAGES Source-specific ingredient and variant review. Every change has an exact PURL-backed excerpt. Prepared-base quantities, unquantified water/titrant, source limitations, chemical identities and prior history are preserved; no taxa-media assertions are added.