Overview
- External ontology
- TOGO:M582 — Desulfatirhabdium Butyrativorans Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M582 Original source: JCM - JCM_M577 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=577
Composition
flowchart LR
CultureMech_009978["desulfatirhabdium_butyrativorans_medium"]
ing0_L__cysteine_HCl_H2O["L--cysteine・HCl・H2O"]
CultureMech_009978 --> ing0_L__cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing0_L__cysteine_HCl_H2O -.-> CHEBI_91248
ing1_N2["N2"]
CultureMech_009978 --> ing1_N2
CHEBI_17997["CHEBI:17997"]
ing1_N2 -.-> CHEBI_17997
ing2_Distilled_water["Distilled water"]
CultureMech_009978 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009978 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009978 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_009978 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_Resazurin["Resazurin"]
CultureMech_009978 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009978 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_NaHCO3["NaHCO3"]
CultureMech_009978 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Na2SO4["Na2SO4"]
CultureMech_009978 --> ing9_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing9_Na2SO4 -.-> CHEBI_32149
ing10_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009978 --> ing10_Yeast_extract__BD_Difco_
ing11_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009978 --> ing11_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing11_Carbon_dioxide_gas -.-> CHEBI_16526
ing12_Nitrogen_gas["Nitrogen gas"]
CultureMech_009978 --> ing12_Nitrogen_gas
ing12_Nitrogen_gas -.-> CHEBI_17997
ing13_crotonic_acid["crotonic acid"]
CultureMech_009978 --> ing13_crotonic_acid
CHEBI_41131["CHEBI:41131"]
ing13_crotonic_acid -.-> CHEBI_41131
ing14_sodium_benzoate["sodium benzoate"]
CultureMech_009978 --> ing14_sodium_benzoate
CHEBI_113455["CHEBI:113455"]
ing14_sodium_benzoate -.-> CHEBI_113455
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
L--cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
10 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Organic compound, Solution |
| 2 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 3 |
Distilled water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 900.0, 100.0] |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.14 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.54 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component |
| 8 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 10 |
Na2SO4
2Na.O4S 142.043 g/mol
|
2.8 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 11 | Yeast extract (BD-Difco) | 0.2 G_PER_L | Properties: Undefined component | |
| 12 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 13 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 14 |
crotonic acid
C4H6O2 86.09 g/mol
|
1.72 G_PER_L |
CHEBI:41131
crotonic acid
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 15 |
sodium benzoate
C7H5O2.Na 144.105 g/mol
|
0.43 G_PER_L |
CHEBI:113455
sodium benzoate
|
Properties: Defined component, Simple component, Organic compound |
Solutions
Na2S・9H2O solutions
Dose: 10 G_PER_L
Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution
Solution A
Dose: 900 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 100 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Trace vitamins solution (see Medium [M278])
Dose: 2 G_PER_L
Cross-reference to Medium M278
Trace element solution (see Medium [M278])
Dose: 1 G_PER_L
Cross-reference to Medium M278
Se/W solution (see Medium [M278])
Dose: 1 G_PER_L
Cross-reference to Medium M278
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.347505Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M582 |
| 2026-01-28T19:48:26.542460Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:44.064258+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T03:09:14.255451Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:20:10.470633Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009978 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:21.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:29.399342+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Desulfatirhabdium Butyrativorans Medium" to "desulfatirhabdium_butyrativorans_medium" for consistent matching |
| 2026-05-16T09:03:19.299919+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:42.749239+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.077920+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |