Overview
- External ontology
- TOGO:M583 — Thermoproteus Medium (B)
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- TOGO M583/JCM Medium 578 prepares Vulcanisaeta Medium from TOGO M291/JCM Medium 297 and adjusts the complete medium to pH 5.5.
Composition
flowchart LR
CultureMech_009979["thermoproteus_medium_b"]
ing0_Resazurin["Resazurin"]
CultureMech_009979 --> ing0_Resazurin
CHEBI_8806["CHEBI:8806"]
ing0_Resazurin -.-> CHEBI_8806
ing1_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_009979 --> ing1_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing1_Na2S_x_9_H2O -.-> CHEBI_76209
ing2_Sulfur__powder_["Sulfur (powder)"]
CultureMech_009979 --> ing2_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing2_Sulfur__powder_ -.-> CHEBI_33403
ing3_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009979 --> ing3_Yeast_extract__BD_Difco_
ing4_H2SO4["H2SO4"]
CultureMech_009979 --> ing4_H2SO4
CHEBI_26836["CHEBI:26836"]
ing4_H2SO4 -.-> CHEBI_26836
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
TOGO M291 lists this component for Vulcanisaeta Medium; TOGO pH variants M583 and M643 adjust the prepared medium to their final pH values. |
| 2 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
TOGO M291 lists this component for Vulcanisaeta Medium; TOGO pH variants M583 and M643 adjust the prepared medium to their final pH values. |
| 3 | Sulfur (powder) | 10 G_PER_L |
CHEBI:33403
elemental sulfur
|
TOGO M291 lists this component for Vulcanisaeta Medium; TOGO pH variants M583 and M643 adjust the prepared medium to their final pH values. |
| 4 | Yeast extract (BD-Difco) | 0.5 G_PER_L | TOGO M291 lists this component for Vulcanisaeta Medium; TOGO pH variants M583 and M643 adjust the prepared medium to their final pH values. | |
| 5 |
H2SO4
H2O4S 98.079 g/mol
|
variable G_PER_L |
CHEBI:26836
sulfuric acid
|
TOGO M291 lists this component for Vulcanisaeta Medium; TOGO pH variants M583 and M643 adjust the prepared medium to their final pH values. |
Preparation
- Prepare Vulcanisaeta Medium from Modified Brock's salt base, yeast extract, sulfur, resazurin, and sodium sulfide under an N2 atmosphere.
- Adjust the complete medium to pH 5.5.
Solutions
Modified Brock's salt base solution
Dose: 1000 ML_PER_L
TOGO M291 uses the Modified Brock's salt base solution from TOGO M156.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.349904Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M583 |
| 2026-03-09T08:04:57.002011+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:10.476527Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009979 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T19:46:29.405395+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Thermoproteus Medium (B)" to "thermoproteus_medium_b" for consistent matching |
| 2026-05-16T09:03:19.306491+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-09-06T00:00:00-07:00 | repair_archaea_togo_score40.py | RESOLVED_ARCHAEA_TOGO_SCORE40_GRAPH | TOGO M583/JCM Medium 578 prepares Vulcanisaeta Medium from TOGO M291/JCM Medium 297 and adjusts the complete medium to pH 5.5. |