Overview
- External ontology
- TOGO:M601 — Clostridium Thermocellum Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M601 Original source: JCM - JCM_M595 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=595
Composition
flowchart LR
CultureMech_010000["clostridium_thermocellum_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010000 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_010000 --> ing1_Yeast_extract
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010000 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_010000 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_Resazurin["Resazurin"]
CultureMech_010000 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010000 --> ing5_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_6H2O -.-> CHEBI_86345
ing6_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_010000 --> ing6_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_7H2O -.-> CHEBI_75836
ing7__NH4_2SO4["(NH4)2SO4"]
CultureMech_010000 --> ing7__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing7__NH4_2SO4 -.-> CHEBI_62946
ing8_K2HPO4_3H2O["K2HPO4・3H2O"]
CultureMech_010000 --> ing8_K2HPO4_3H2O
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4_3H2O -.-> CHEBI_131527
ing9_Cellulose__Avicel_or_MN_300_["Cellulose (Avicel or MN 300)"]
CultureMech_010000 --> ing9_Cellulose__Avicel_or_MN_300_
ing10_Glutathione["Glutathione"]
CultureMech_010000 --> ing10_Glutathione
CHEBI_16856["CHEBI:16856"]
ing10_Glutathione -.-> CHEBI_16856
ing11_Sodium____glycerophosphate["Sodium β--glycerophosphate"]
CultureMech_010000 --> ing11_Sodium____glycerophosphate
ing12_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010000 --> ing12_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing12_Carbon_dioxide_gas -.-> CHEBI_16526
ing13_Nitrogen_gas["Nitrogen gas"]
CultureMech_010000 --> ing13_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing13_Nitrogen_gas -.-> CHEBI_17997
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 2 | Yeast extract | 4.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.13 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.43 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component |
| 6 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.6 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 7 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 8 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound |
| 9 |
K2HPO4・3H2O
HO4P.2K 174.174 g/mol
|
7.2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 10 | Cellulose (Avicel or MN 300) | 10 G_PER_L | Role: Carbon source; Properties: Simple component, Organic compound, Defined component | |
| 11 |
Glutathione
C10H17N3O6S 307.328 g/mol
|
0.25 G_PER_L |
CHEBI:16856
glutathione
|
Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component |
| 12 | Sodium β--glycerophosphate | 6 G_PER_L | Role: Mineral source; Properties: Organic compound, Simple component, Defined component | |
| 13 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 14 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.384984Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M601 |
| 2026-01-28T19:48:24.321768Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-13T06:20:10.624092Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010000 |
| 2026-03-17T06:30:16.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:29.551093+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Clostridium Thermocellum Medium" to "clostridium_thermocellum_medium" for consistent matching |
| 2026-05-16T09:03:19.451054+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:42.906558+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.128081+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |